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GSK-3beta S9A (Human)/pCS2+
(Plasmid #16334)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16334 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    glycogen synthase kinase-3 beta
  • Alt name
    GSK3b
  • Alt name
    GSK-3beta
  • Alt name
    S9A, NPt
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Mutation
    GSK-3 beta with serine-9 to alanine point mutation
  • Entrez Gene
    GSK3B
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

GSK-3beta with ser9->ala mutation blocks N terminal phosphorylation. For sense transcripts, cut with NotI and transcribe with SP6.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GSK-3beta S9A (Human)/pCS2+ was a gift from Peter Klein (Addgene plasmid # 16334 ; http://n2t.net/addgene:16334 ; RRID:Addgene_16334)