177,004 results
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Plasmid#118755PurposeBinary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.DepositorInsertTobacco mosaic virus
ExpressionPlantPromoterCauliflower mosaic virus 35SAvailable SinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1Alpha-Tet-on 3G
Plasmid#118592PurposeExpresses Tet-on 3G from the EF1Alpha promoterDepositorInsertTet-on 3G
UseLentiviralExpressionMammalianPromoterEF1AlphaAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI
Plasmid#117006PurposeExpresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
tetO.Nfia.Blast
Plasmid#117270PurposeExpresses Nfia and blasticidin resistance gene under control of tetO sequence.DepositorAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-DRGFP
Plasmid#113193PurposeDR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.DepositorInsertDR-GFP
UseDonor construct with t2a-hygromycin for gene trap…ExpressionMammalianAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.eGFP.WPRE.rBG (AAV8)
Viral Prep#105542-AAV8PurposeReady-to-use AAV8 particles produced from pENN.AAV.CB7.CI.eGFP.WPRE.rBG (#105542). In addition to the viral particles, you will also receive purified pENN.AAV.CB7.CI.eGFP.WPRE.rBG plasmid DNA. CB7-driven eGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCB7TagsEGFPAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-CMV-mCherry-T2A-GFP
Plasmid#109427PurposeA lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.DepositorInsertmCherry T2A GFP
UseLentiviralPromoterCMVAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-P2A-Puro
Plasmid#110837PurposeLentiviral vector for expression of Cas9 in mammalian cells (codon optimized)DepositorInsertCas9
UseLentiviralMutationWTPromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-Tom20-Halo7Tag
Plasmid#111135PurposeTom20 with selflabeling tag, e.g. for fluorescence microscopyDepositorInsertReceptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)
TagsHalo7 TagExpressionMammalianPromoterCMVAvailable SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-csGBP
Plasmid#108875PurposeExpression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)DepositorInsertmKate2-P2A-APEX2-csGBP
ExpressionMammalianAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
TagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.18 CMV-Luc
Plasmid#100984PurposeCMV luciferase reporter - firefly luciferase driven by CMV promoterDepositorInsertCMV
ExpressionMammalianAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVET-HA-K-RasG12V-IRES-GFP
Plasmid#107140PurposeA bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFPDepositorInsertHA-tagged Kras4B(G12V) and GFP (KRAS Human)
UseLentiviralExpressionMammalianPromoterhEF-1alphaAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHIV-H2B-eGFP
Plasmid#91776PurposeExpresses H2B protein tagged with eGFPDepositorInsertHuman Histone H2B / eGFP (H2BC11 Human)
UseLentiviralTagseGFPExpressionMammalianPromoterEF1aAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFr.WPRE.SV40 (AAV5)
Viral Prep#98929-AAV5PurposeReady-to-use AAV5 particles produced from pAAV.hSyn.iGluSnFr.WPRE.SV40 (#98929). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFr.WPRE.SV40 plasmid DNA. Synapsin-driven, iGluSnFr glutamate sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceFeb. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
PVS10
Plasmid#104398Purposepolycistronic vector for expression of E. coli core RNAPDepositorTagsHis6ExpressionBacterialPromoterT7Available SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mGFPmut2
Plasmid#103980PurposePlasmid encoding mGFPmut2DepositorInsertmGFPmut2
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S65A, V68L, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP4B_LAP
Plasmid#101850PurposeTet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCharged Multivesicular Body Protein 4B (CHMP4B Human)
UseGateway cloning, flp-frt recombination, tet-induc…TagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianPromoterCMVAvailable SinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only