PurposeFor analysis of PH domain localization in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71378||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
MutationPleckstrin homology (PH) domain; aa 247-399
Entrez GeneCyth3 (a.k.a. AI648983, ARNO3, CLM3, Grp1, Pscd3)
- Promoter CMV
/ Fusion Protein
- EGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Grp1-PH pEGFP-C1 was a gift from Mark Lemmon (Addgene plasmid # 71378 ; http://n2t.net/addgene:71378 ; RRID:Addgene_71378)
For your References section:Specificity and promiscuity in phosphoinositide binding by pleckstrin homology domains. Kavran JM, Klein DE, Lee A, Falasca M, Isakoff SJ, Skolnik EY, Lemmon MA. J Biol Chem. 1998 Nov 13;273(46):30497-508. PubMed 9804818