Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

mouse deltaPE Oct4-GFP transgenic reporter
(Plasmid #52382)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52382 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBS KS+
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 22128
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    GFP pA
  • Species
    Synthetic
  • Promoter Oct4

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Oct4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    18000
  • Mutation
    deleted -300 - -1300bp fragment upstream from Oct4 ATG
  • Entrez Gene
    Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    PGK-gb2-Neo
  • Species
    Synthetic
  • Insert Size (bp)
    1516
  • Promoter PGK+gb2

Cloning Information for Gene/Insert 3

Resource Information

Depositor Comments

This construct is modified pGOF18 from Young Young Il Yeom et al.,Development 122, 000-000 (1996) 881. We have introduced neo selection cassette at the 3' end of the cloned Oct4 locus.

Please note that there are several mismatches in between Addgene's quality control and the depositor's sequence. The mismatches do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mouse deltaPE Oct4-GFP transgenic reporter was a gift from Jacob Hanna (Addgene plasmid # 52382 ; http://n2t.net/addgene:52382 ; RRID:Addgene_52382)
  • For your References section:

    Derivation of novel human ground state naive pluripotent stem cells. Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D, Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, Novershtern N, Hanna JH. Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30. 10.1038/nature12745 PubMed 24172903