mouse Oct4-GFP GOF18 transgenic reporter
PurposeBAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60527||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBS KS+
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 21615
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)970
- Promoter Oct4
- Cloning method Unknown
- 5′ sequencing primer TTGTCCTGTCCAGACGTCCC
- 3′ sequencing primer TACCTCTGAGCCTGGTCCGA (Common Sequencing Primers)
Terms and Licenses
This construct is modified pGOF18 from Young Young Il Yeom et al.,Development 122, 000-000 (1996) 881.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mouse Oct4-GFP GOF18 transgenic reporter was a gift from Jacob Hanna (Addgene plasmid # 60527)
For your References section:Derivation of novel human ground state naive pluripotent stem cells. Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D, Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, Novershtern N, Hanna JH. Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30. 10.1038/nature12745 PubMed 24172903
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.