PurposeExpresses wild type human PTPN14 in mammalian cells, N-terminus V5 tag
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61003||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 9061
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)3561
Entrez GenePTPN14 (a.k.a. CATLPH, PEZ, PTP36)
- Promoter CMV
/ Fusion Protein
- V5 tag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDNA3-V5-PTPN14-wild type was a gift from Jianmin Zhang (Addgene plasmid # 61003 ; http://n2t.net/addgene:61003 ; RRID:Addgene_61003)
For your References section:PTPN14 forms a complex with Kibra and LATS1 proteins and negatively regulates the YAP oncogenic function. Wilson KE, Li YW, Yang N, Shen H, Orillion AR, Zhang J. J Biol Chem. 2014 Aug 22;289(34):23693-700. doi: 10.1074/jbc.M113.534701. Epub 2014 Jul 14. 10.1074/jbc.M113.534701 PubMed 25023289