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(Plasmid #111135)


Item Catalog # Description Quantity Price (USD)
Plasmid 111135 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Covalys, NEB
  • Backbone size w/o insert (bp) 5325
  • Total vector size (bp) 6570
  • Modifications to backbone
    original snap-Tag substituted by Halo7-Tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Receptor of tranlocase of outer mitochondrial membrane Tom20
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    TOMM20 (a.k.a. MAS20, MOM19, TOM20)
  • Promoter CMV
  • Tag / Fusion Protein
    • Halo7 Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ATGGTGGGTCGGAACAGCGCCAT
  • 3′ sequencing primer TTCCACATCATCTTCAGCCAAG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEMS-Tom20-Halo7Tag was a gift from Karin Busch (Addgene plasmid # 111135 ; ; RRID:Addgene_111135)
  • For your References section:

    Nanoscale organization of mitochondrial microcompartments revealed by combining tracking and localization microscopy. Appelhans T, Richter CP, Wilkens V, Hess ST, Piehler J, Busch KB. Nano Lett. 2012 Feb 8;12(2):610-6. doi: 10.1021/nl203343a. Epub 2012 Jan 13. 10.1021/nl203343a PubMed 22201267