PurposeCan be used to generate AAV virus that will express mScarlet in the presence of Cre
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||99280||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backboneAAV pCAG-FLEX-tdTomato-WPRE (Addgene #51503)
Backbone manufacturerHongkui Zeng/Allen Institute For Brain Science
- Backbone size w/o insert (bp) 5719
- Total vector size (bp) 6440
Modifications to backboneA fragment containing reverse-complemented mScarlet was swapped into replace tdTomato. Total AAV packaging size (including ITRs and DNA in between) is 3843 bp.
Vector typeAAV, Cre/Lox
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Alt namemonomeric Scarlet
Alt namemScarlet red fluorescent protein
Insert Size (bp)696
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site FseI (not destroyed)
- 5′ sequencing primer gcaacgtgctggttattgtg
- 3′ sequencing primer TAGAAGGCACAGTCGAGG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The stop codon for mRuby3 ends 24 bp downstream of the gene, adding an additional 8 amino acids to the protein. However, this is not expected to impact the function of the protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV pCAG-FLEX-mScarlet-WPRE was a gift from Rylan Larsen (Addgene plasmid # 99280 ; http://n2t.net/addgene:99280 ; RRID:Addgene_99280)