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Double UP mScarlet to mNeon
(Plasmid #125139)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 125139 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    Removed Not1 site in mScarlet

Resource Information

Depositor Comments

Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mScarlet can act as an internal control to mNeon for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mNeon through use of a mcs after the P2A.

Please visit https://www.biorxiv.org/content/early/2019/03/08/571323.full.pdf for BioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Double UP mScarlet to mNeon was a gift from Erik Dent (Addgene plasmid # 125139 ; http://n2t.net/addgene:125139 ; RRID:Addgene_125139)
  • For your References section:

    Double UP: efficient in utero electroporation via internal controls. Taylor RJ, Carrington J, Taylor KL, Richters KE, Gerlach LR, Dent EW. BioRxiv, March 8, 2019 10.1101/571323