Double UP mScarlet to mNeon
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||125139||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6734
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
MutationRemoved Not1 site in mScarlet
Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mScarlet can act as an internal control to mNeon for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mNeon through use of a mcs after the P2A.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Double UP mScarlet to mNeon was a gift from Erik Dent (Addgene plasmid # 125139 ; http://n2t.net/addgene:125139 ; RRID:Addgene_125139)
For your References section:Double UP: A Dual Color, Internally Controlled Platform for in utero Knockdown or Overexpression. Taylor RJ, Carrington J, Gerlach LR, Taylor KL, Richters KE, Dent EW. Front. Mol. Neurosci. 13:82 10.3389/fnmol.2020.00082