Double UP mNeon to mScarlet
Purpose(Empty Backbone) Natively produces mNeon, in the presence of Cre will be recombined to produce mScarlet.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||125134||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6734
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
MutationRemoved Not1 site in mScarlet
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A.
Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Double UP mNeon to mScarlet was a gift from Erik Dent (Addgene plasmid # 125134 ; http://n2t.net/addgene:125134 ; RRID:Addgene_125134)
For your References section:Double UP: A Dual Color, Internally Controlled Platform for in utero Knockdown or Overexpression. Taylor RJ, Carrington J, Gerlach LR, Taylor KL, Richters KE, Dent EW. Front Mol Neurosci. 2020 May 20;13:82. doi: 10.3389/fnmol.2020.00082. eCollection 2020. 10.3389/fnmol.2020.00082 PubMed 32508591