164,884 results

1. pCW-HA-hRb-WT-puro

   Plasmid

   #212672

   Purpose

   Express tagged hRb

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-RPS6 (pDD1902)

   Plasmid

   #52913

   Purpose

   contains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.

   Depositor

   Dirk Dittmer

   Article

   Chen et al J Virol. 2011 Sep;85(18):9495-505. doi:

   Insert

   HAS6 (RPS6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLARRYv2-EGFP

   Plasmid

   #233208

   Purpose

   Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP marker

   Depositor

   Alejo Rodriguez-Fraticelli

   Article

   Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2CT-His-MBP-Lbu_C2c2_WT

   Plasmid

   #83482

   Purpose

   Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_WT
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

   Plasmid

   #194284

   Purpose

   EF1a-dCas9-KRAB-GFP with nontarget sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cav-GA

   Plasmid

   #209880

   Purpose

   Dimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP A
   

   Use

   Tags

   CAVIN1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. G1A

   Plasmid

   #24690

   Depositor

   Jeanne Lawrence

   Article

   Clemson et al J Cell Biol. 1996 Feb . 132(3):259-75.

   Insert

   human XIST (XIST Human)
   

   Use

   Plasmid cloning vector

   Tags

   Expression

   Mutation

   Genomic insert. Includes introns 5-8.

   Promoter

   Available since

   Jan. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. Inzolia library in pRDA_550

   Pooled Library

   #209552

   Purpose

   The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Use

   CRISPR and Lentiviral

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

9. pET28C-SARS-CoV-2-Nucleocapsid

   Plasmid

   #204402

   Purpose

   E. coli expression of full-length SARS-CoV-2 nucleocapsid

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-N-Puro-mStayGold

    Plasmid

    #227250

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV Retrograde)

    Viral Prep

    #26966-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

    Promoter

    EF1a

    Tags

    EYFP (Cre-dependent)

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

12. pCBC-DT3T4

    Plasmid

    #50592

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T3, gRNA scaffold, U6-1tplus, U6-26p, T4
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO-CreERT2 stuffer v3

    Plasmid

    #158046

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. p6XHis_NLS-SaCas9

    Plasmid

    #101086

    Purpose

    Express SaCas9 in bacteria with a 6xHis tag for purification

    Depositor

    Rick Tarleton

    Article

    Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

    Insert

    CRISPR-associated protein Cas9/Csn1
    

    Use

    CRISPR

    Tags

    TrxA-6xHis-STag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid

    #107055

    Purpose

    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE

    Plasmid

    #198516

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS mouse ER81 [TJ#197]

    Plasmid

    #16282

    Depositor

    Thomas Jessell

    Insert

    ER81 (Etv1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pNeonGreen-C1-Nir1-LNS2

    Plasmid

    #220083

    Purpose

    high affinity PA biosensor with green fluorophore

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Use

    Tags

    NeonGreen-GGSGGM

    Expression

    Mammalian

    Mutation

    amino acids 613-897

    Promoter

    CMV

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TCARZ-CMV-GFP-PAmin-GF3-R4A22

    Plasmid

    #203768

    Purpose

    For production of template RNA for use in PRINT, GFP under CMV promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for GFP
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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