|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15726||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6400
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSmad3 shRNA
SpeciesH. sapiens (human)
Insert Size (bp)60
Entrez GeneSMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer H1 (Common Sequencing Primers)
The following oligos were cloned into pRetrosuper-Puro digested with BglII and HindIII.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRetroSuper Smad3 was a gift from Joan Massague (Addgene plasmid # 15726 ; http://n2t.net/addgene:15726 ; RRID:Addgene_15726)
For your References section:Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. He W, Dorn DC, Erdjument-Bromage H, Tempst P, Moore MA, Massague J. Cell. 2006 Jun 2. 125(5):929-41. 10.1016/j.cell.2006.03.045 PubMed 16751102
Map uploaded by the depositor.