Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15723||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameSmad3 shRNA
SpeciesH. sapiens (human)
Insert Size (bp)450
Entrez GeneSMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer H1 (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The following oligos were first cloned into pRetrosuper-Puro digested with BglII and HindIII.
Next, the AgeI/XhoI fragments from shRNA sequence-containing pRetrosuper-Puro plasmids were cloned into the AgeI/XhoI site of pRetrosuper-GFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRetroSuper-GFP Smad3 was a gift from Joan Massague (Addgene plasmid # 15723 ; http://n2t.net/addgene:15723 ; RRID:Addgene_15723)
For your References section:Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. He W, Dorn DC, Erdjument-Bromage H, Tempst P, Moore MA, Massague J. Cell. 2006 Jun 2. 125(5):929-41. 10.1016/j.cell.2006.03.045 PubMed 16751102
Map uploaded by the depositor.