287,729 results

1. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

   Plasmid

   #67977

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
   

   Use

   Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDest-mCherry-N1

   Plasmid

   #31907

   Depositor

   Robin Shaw

   Article

   Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDest-mCherry-N1

   Plasmid

   #31907

   Depositor

   Robin Shaw

   Article

   Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRH-1346 U6-B2M sgRNA Gag-pol v2

   Plasmid

   #201917

   Purpose

   Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-pol
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Expression

   Mammalian

   Promoter

   CAG and Human U6

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

   Plasmid

   #199371

   Purpose

   Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-ALFA nanobody
   

   Tags

   14xHis-Avi

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T5-LacO

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-AKAR4

   Plasmid

   #61619

   Purpose

   FRET-based biosensor for monitoring PKA activity

   Depositor

   Jin Zhang

   Article

   Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

   Insert

   AKAR4
   

   Tags

   Cerulean and cpVenus[E172]

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-AKAR4

   Plasmid

   #61619

   Purpose

   FRET-based biosensor for monitoring PKA activity

   Depositor

   Jin Zhang

   Article

   Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

   Insert

   AKAR4
   

   Tags

   Cerulean and cpVenus[E172]

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEx-ER-TurboID

   Plasmid

   #160857

   Purpose

   Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Has Service

   AAV8

   Insert

   ER TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Promoter

   CAG

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEx-ER-TurboID

   Plasmid

   #160857

   Purpose

   Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Has Service

   AAV8

   Insert

   ER TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Promoter

   CAG

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Inzolia library in pRDA_550

    Pooled Library

    #209552

    Purpose

    The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

    Depositor

    John Doench

    Article

    Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

11. Flag-mPIAS1

    Plasmid

    #15206

    Depositor

    Ke Shuai

    Article

    Liu et al Proc Natl Acad Sci U S A. 1998 Sep 1. 95

    Insert

    PIAS1 (Pias1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-mPIAS1

    Plasmid

    #15206

    Depositor

    Ke Shuai

    Article

    Liu et al Proc Natl Acad Sci U S A. 1998 Sep 1. 95

    Insert

    PIAS1 (Pias1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)-ExRai-AKAR2

    Plasmid

    #161753

    Purpose

    Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-ExRai-AKAR2

    Plasmid

    #161753

    Purpose

    Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-GFP-MDC1 (770-7)

    Plasmid

    #26284

    Depositor

    Eric Campeau

    Insert

    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Entry vector

    Tags

    GFP

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-GFP-MDC1 (770-7)

    Plasmid

    #26284

    Depositor

    Eric Campeau

    Insert

    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Entry vector

    Tags

    GFP

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. CL20_mEGFP_TMPRSS2_ETV4

    Plasmid

    #205945

    Purpose

    Express mEGFP-tagged fusion protein, TMPRSS2_ETV4 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    TMPRSS2_ETV4 (TMPRSS2, ETV4 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAW12.lentiguide.GFP

    Plasmid

    #104374

    Purpose

    GFP vector for cloning in guides

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAW12.lentiguide.GFP

    Plasmid

    #104374

    Purpose

    GFP vector for cloning in guides

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCIpA102-G-HLA-A2_GFP

    Plasmid

    #85162

    Purpose

    for mRNA expression of HLA.A2-EGFP

    Depositor

    Sébastien Walchli, Tone Fredsvik Gregers

    Article

    Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

    Insert

    HLA.A2 (HLA-A Human)
    

    Use

    Mrna expression

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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