A bistable inhibitory OptoGPCR for multiplexed optogenetic control of neural circuits.
Wietek J, Nozownik A, Pulin M, Saraf-Sinik I, Matosevich N, Malan D, Brown BJ, Dine J, Levy R, Litvin A, Regev N, Subramaniam S, Bitton E, Benjamin A, Copits BA, Sasse P, Rost BR, Schmitz D, Soba P, Nir Y, Wiegert JS, Yizhar O
bioRxiv. 2023 Jul 2:2023.07.01.547328. doi: 10.1101/2023.07.01.547328. Preprint. PubMed Article
Wietek J, Nozownik A, Pulin M, Saraf-Sinik I, Matosevich N, Malan D, Brown BJ, Dine J, Levy R, Litvin A, Regev N, Subramaniam S, Bitton E, Benjamin A, Copits BA, Sasse P, Rost BR, Schmitz D, Soba P, Nir Y, Wiegert JS, Yizhar O
bioRxiv. 2023 Jul 2:2023.07.01.547328. doi: 10.1101/2023.07.01.547328. Preprint. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198507 | pcDNA3.1-CMV-PdCO-mScarlet | Expresses optimized PdCO in frame with mScarlet under control of CMV promotor. |
| 198508 | pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE | Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor. |
| 198509 | pAAV-hSyn-SIO-PdCO-mScarlet-WPRE | Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination. |
| 198510 | pAAV_hSyn-PdCO-mScarlet-WPRE | Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor. |
| 198511 | pAAV_hSyn-DIO-PdCO-mScarlet-WPRE | Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination. |
| 198512 | pcDNA3.1-CMV-PdCO-EGFP | Expresses optimized PdCO in frame with EGFP under control of CMV promotor. |
| 198513 | pAAV_hSyn-PdCO-EGFP-WPRE | Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor. |
| 198514 | pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE | Expresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor. |
| 198515 | pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE | Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination. |
| 198516 | pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE | Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination. |
| 202198 | pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE | Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotor |
| 202199 | pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE | Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination |