167,808 results
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pD649-HAsp-HLA-E-COMP5AP-AviTag-9xHis
Plasmid#157396PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB-CMPE-ePPEplus
Plasmid#205246PurposeFor plant prime editing in wheat plantsDepositorInsertCsy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable SinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVTRup
Plasmid#101362PurposeHighly efficient in vitro transcription. Empty backbone.DepositorInsert5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
UseFor cloning a gene for in vitro transcription pur…PromoterT7Available SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgRosa26
Plasmid#159914PurposeMutagenesis of Rosa26 with SauCas9DepositorInsertRosa26 gRNA (Gt(ROSA)26Sor Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
dCas12j3 VPR
Plasmid#188517PurposeExpresses FLAG tagged dCas12j3 VPRDepositorInsertdCas12j3
UseCRISPR and LentiviralExpressionMammalianMutationD413APromoterEF1aAvailable SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Luc-T20
Plasmid#177941PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
ExpressionMammalianAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRK246
Plasmid#173741Purposedonor plasmid for SapTrap assemblyDepositorInsertwrt-2 promoter
UseOtherAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD373
Plasmid#137021PurposeSapTrap GFP-GLO donorDepositorInsertC. elegans GLO-optimized GFP
UseCRISPR; Saptrap donor for cloning repair templat…TagsC. elegans GLO-optimized GFPExpressionWormAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD
Plasmid#169818PurposeExpresses C-terminal flag-tagged CAD in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCCC -> AGTC silent mutations at nt527-530 eli…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVtight Puro DEST (w768-1)
Plasmid#26430DepositorTypeEmpty backboneUseLentiviral; Tet-on advanced destination vectorExpressionMammalianAvailable SinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEB2-E2-Crimson
Plasmid#104010PurposePlasmid encoding E2-CrimsonDepositorInsertE2-Crimson
UseLow copyExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpen-DpnIR
Plasmid#165529PurposeUsed to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)DepositorInsertDpnI
UseSynthetic BiologyAvailable SinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CIBN-CAAX
Plasmid#79574Purposeexpression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targetingDepositorInsertCIBN (CIB1 Mustard Weed)
TagsC-terminal CAAX-boxExpressionMammalianMutationK93A, R94A, K106A, K107A (please see depositor co…PromoterCMVAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLS714
Plasmid#188886PurposeMiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119DepositorInsertPmex-5::Cas9
ExpressionWormMutationC. elegans codom optimization and intron additionAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-ccdB
Plasmid#14147DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG/GFP Dest
Plasmid#74281PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseMouse TargetingAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9
Plasmid#140237PurposeLentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3xFLAGExpressionMammalianPromoterEF-1a coreAvailable SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_ManII-SBP-EGFP
Plasmid#65258Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseLentiviralTagsEGFP and Streptavidin Binding Protein (SBP)Mutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFD6
Plasmid#73915Purposetissue-specific multiplex gRNA plasmidDepositorInsert:tRNA:gRNA:tRNA:gRNA:tRNA
UseCRISPRExpressionInsectPromoterUAS-DSCPAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only
