183,825 results

1. pHIV-Zsgreen

   Plasmid

   #18121

   Depositor

   Zena Werb, Bryan Welm

   Article

   Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

   Insert

   Zsgreen
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIV-Zsgreen

   Plasmid

   #18121

   Depositor

   Zena Werb, Bryan Welm

   Article

   Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

   Insert

   Zsgreen
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-SKII-3XHA-HALO-NAT

   Plasmid

   #188931

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-HALO-NAT
   

   Expression

   Yeast

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT-TOgw-C-mCherry

   Plasmid

   #223952

   Purpose

   Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Karla Feijs, Roko Žaja

   Type

   Empty backbone

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-PDGFRb-IRES2-EGFP

   Plasmid

   #204354

   Purpose

   Expresses wild-type PDGFRb gene.

   Depositor

   Hirofumi Nakatomi

   Article

   Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

   Insert

   Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
   

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR CD19 tandem CAR

   Plasmid

   #113019

   Purpose

   for lentivirus production of CD19 tandem CAR

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   CD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Human, Synthetic, Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

   Plasmid

   #180012

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; HE1A: BFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-28a(+)-T7-OmpA-MNaseA-6xHis

   Plasmid

   #214808

   Purpose

   Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

   Depositor

   Peter Sarin

   Article

   Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

   Insert

   OmpA-Micrococcal nuclease A
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV1)

   Viral Prep

   #105545-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   HI

   Tags

   EGFP

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-HyPer7-MEM

    Plasmid

    #136465

    Purpose

    Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Tags

    Farnselation tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME phluorin2-gpi

    Plasmid

    #73793

    Purpose

    ratiometric pHluorin2 with a gpi linkage sequence in a middle entry clone

    Depositor

    David Raible

    Article

    Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

    Insert

    ratiometric pHluorin2-gpi
    

    Use

    Middle entry clone

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.hSyn.iGluSnFR3.v857.PDGFR (AAV1)

    Viral Prep

    #178329-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.hSyn.iGluSnFR3.v857.PDGFR (#178329). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFR3.v857.PDGFR plasmid DNA. Synapsin-driven, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3-HA-human MYCN

    Plasmid

    #74163

    Purpose

    Mammalian expression of human MycN

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MycN (MYCN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-SaCas9

    Plasmid

    #178901

    Purpose

    SaCas9 (Cas9 from Staphylococcus aureus)

    Depositor

    Hendrik Milting

    Article

    Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

    Insert

    SaCas9
    

    Tags

    His6-Tag, bipartite nuclear localization signal f…

    Expression

    Bacterial

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. actc1b-2PH-PLCd-mKate2

    Plasmid

    #109500

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-2PH-PLCd-mKate2
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Butelase AY

    Plasmid

    #221847

    Purpose

    Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

    Insert

    Butelase 1 ligase
    

    Expression

    Bacterial

    Mutation

    Val237Ala, Thr238Tyr

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFH2.22_mCherry-MS2x8

    Plasmid

    #205537

    Purpose

    Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Hy_pMT Flag-UAP56 WT

    Plasmid

    #110125

    Purpose

    Expresses Flag-tagged human UAP56 (DDX39B)

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    UAP56 (DDX39B Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-ISceID44A

    Plasmid

    #59424

    Purpose

    Expresses a catalytically inactive form of the endonuclease ISceI

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    endonuclease ISceI mutant (I-SceI )
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed aspartate 44 to alanine (D44A)

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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