212,868 results

1. CDH5-AbR-GFP

   Plasmid

   #122970

   Purpose

   Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.

   Depositor

   Budd A. Tucker

   Inserts

   CDH5p
   CopGFP
   Zeocin
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF6-Lck-TurboID

   Plasmid

   #159433

   Purpose

   expresses Lck-TurboID in mammalian cells

   Depositor

   Arthur Salomon

   Article

   Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

   Inserts

   Lck (LCK Human)
   TurboID (BirA mutant)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   EF-1a

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3XFlag-CAPN1

   Plasmid

   #60941

   Purpose

   Expresses calpain1 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Calpain1 (CAPN1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3XFlag-CAPN1

   Plasmid

   #60941

   Purpose

   Expresses calpain1 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Calpain1 (CAPN1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF6a-CD28-PafA

   Plasmid

   #113400

   Purpose

   Expresses CD28-PafA in mammalian cell

   Depositor

   Min Zhuang

   Article

   Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

   Insert

   CD28-PafA
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. NNMT

   Plasmid

   #40734

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NNMT (NNMT Human)
   

   Use

   Tags

   His Thrombin

   Expression

   Bacterial

   Mutation

   K100A:E101A:E103A

   Promoter

   Available since

   Oct. 18, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

7. NNMT

   Plasmid

   #40734

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NNMT (NNMT Human)
   

   Use

   Tags

   His Thrombin

   Expression

   Bacterial

   Mutation

   K100A:E101A:E103A

   Promoter

   Available since

   Oct. 18, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pDONR221_SLC6A15

   Plasmid

   #131877

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC6A15 (SLC6A15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCAS

   Plasmid

   #60847

   Purpose

   Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

   Depositor

   Jamie Cate

   Article

   Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

   Inserts

   S. pyogenese Cas9
   RNA pol III promoter (tRNA-Tyr)
   hepatitis delta virus ribozyme, genomic
   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS/His8 and TTT 3' extension prior to sgRNA

   Expression

   Bacterial and Yeast

   Mutation

   L4 is UUCG tetraloop and guide targets LYP1 (CATA…

   Promoter

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAS

    Plasmid

    #60847

    Purpose

    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

    Depositor

    Jamie Cate

    Article

    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

    Inserts

    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS/His8 and TTT 3' extension prior to sgRNA

    Expression

    Bacterial and Yeast

    Mutation

    L4 is UUCG tetraloop and guide targets LYP1 (CATA…

    Promoter

    Available since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAd5-B6 ∆E3-GFP

    Plasmid

    #179201

    Purpose

    Block 6 with deletion in E3 and insertion of GFP expression cassette

    Depositor

    Fred Bunz

    Article

    Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

    Insert

    Modified block 6, with deletion in E3 and insertion of GFP expression cassette
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4xCSL-luciferase

    Plasmid

    #41726

    Depositor

    Raphael Kopan

    Article

    Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

    Insert

    4xCSL binding sites
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains a multimerized high affinity CSL binding…

    Promoter

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4xCSL-luciferase

    Plasmid

    #41726

    Depositor

    Raphael Kopan

    Article

    Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

    Insert

    4xCSL binding sites
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains a multimerized high affinity CSL binding…

    Promoter

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF3529

    Plasmid

    #145005

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    VDR (VDR Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

    Plasmid

    #185918

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pdCas9- AsiA_m2.1

    Plasmid

    #158065

    Purpose

    Expressing evolved CasTA of dCas9 fused AsiA variant 2.1

    Depositor

    Harris Wang

    Article

    Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

    Insert

    dCas9-AsiA_m2.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. DR274

    Plasmid

    #42250

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Worm

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. DR274

    Plasmid

    #42250

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Worm

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICH41780

    Plasmid

    #48019

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pICH41780

    Plasmid

    #48019

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge