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(Plasmid #110312)


Item Catalog # Description Quantity Price (USD)
Plasmid 110312 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2685
  • Total vector size (bp) 3519
  • Modifications to backbone
    In-Fusion cloning was used for construction of this plasmid.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    TR-TUBE was expressed in E.coli Rosetta2 (DE3) with 0.2 mM IPTG for 15 h at 22°C.
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    4 tandem repeats of the trypsin-resistant UBQLN1 UBA domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    All Arg residues in the UBA domain are mutated to Ala.
  • GenBank ID
  • Entrez Gene
    UBQLN1 (a.k.a. DA41, DSK2, PLIC-1, UBQN, XDRP1)
  • Promoter T7
  • Tag / Fusion Protein
    • N-terminal His6-T7 tag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer CTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Nucleotide sequence is not identical to NM_013438.4, but amino acid sequence is as described in Gene/Insert: Mutation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET-4xTR-TUBE was a gift from Yasushi Saeki (Addgene plasmid # 110312 ; ; RRID:Addgene_110312)
  • For your References section:

    Ub-ProT reveals global length and composition of protein ubiquitylation in cells. Tsuchiya H, Burana D, Ohtake F, Arai N, Kaiho A, Komada M, Tanaka K, Saeki Y. Nat Commun. 2018 Feb 6;9(1):524. doi: 10.1038/s41467-018-02869-x. 10.1038/s41467-018-02869-x [pii] PubMed 29410401