207,825 results

1. iF65 PB-FrtStopFrt-Zim3-dCas9-mScarlet-hygro

   Plasmid

   #204730

   Purpose

   Frt-inducible CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-MAP2K1

   Plasmid

   #23406

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiGuide-Hygro-dTomato

   Plasmid

   #99376

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-dTomato
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. phL1A-pcDNA3

   Plasmid

   #12307

   Depositor

   Vance Lemmon

   Article

   Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

   Insert

   L1CAM (L1CAM Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHBintT

   Plasmid

   #48134

   Purpose

   Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-HA-hIFITM1

   Plasmid

   #58399

   Purpose

   Expressed human IFITM1 with an N-terminal HA tag in mammalian cells

   Depositor

   Jacob Yount, Howard Hang

   Article

   Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

   Insert

   IFITM1 (IFITM1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-synaptophysin-Lime

   Plasmid

   #201016

   Purpose

   Expresses Lime in synaptic vesicle lumen by fusion to synaptophysin

   Depositor

   Jaime de Juan-Sanz

   Article

   Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

   Insert

   Synaptophysin-Lime (Syp Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-RapGAP

   Plasmid

   #118324

   Purpose

   Expression of RapGAP

   Depositor

   Philip Stork

   Article

   Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

   Insert

   RapGAP
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-mCherry

   Plasmid

   #41583

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-mStayGold-Puro

    Plasmid

    #227253

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKB225

    Plasmid

    #170015

    Purpose

    Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Peptide display protein with no C-terminal peptide
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4XCLEAR-luciferase reporter

    Plasmid

    #66800

    Purpose

    Reporter for activity of the CLEAR network

    Depositor

    Albert La Spada

    Article

    Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

    Insert

    4XCLEAR
    

    Use

    Luciferase

    Promoter

    Four CLEAR elements (GTCACGTGAC) in tandem derive…

    Available Since

    Nov. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid

    #129593

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Tags

    6xHis-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.2/v5-DEST hGlut4

    Plasmid

    #18087

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    Glut4 (SLC2A4 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRT043-CLYBL-DDdcas9-VPH

    Plasmid

    #158091

    Purpose

    Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

    Depositor

    Martin Kampmann

    Article

    Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

    Insert

    CLYBL-CAG-DDdCas9VPH-T2A-GFP
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2-shP53

    Plasmid

    #124261

    Purpose

    Expresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.

    Depositor

    Maria Castro

    Article

    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

    Insert

    shP53
    

    Expression

    Mammalian

    Available Since

    April 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3.1-hDAT

    Plasmid

    #32810

    Depositor

    Susan Amara

    Insert

    DAT (SLC6A3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-Puro-TDP-43-WT

    Plasmid

    #133753

    Purpose

    Wild type human TDP-43 in pLVX-Puro lentiviral vector

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. HBx

    Plasmid

    #65463

    Purpose

    expresses HBx in mammalian cells

    Depositor

    Wang-Shick Ryu

    Article

    Cha et al Hepatology. 2004 Jun;39(6):1683-93.

    Insert

    HBV X protein
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-Neo-Bam p53 R175H

    Plasmid

    #16436

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R175H (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    CMV

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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