164,868 results

1. pFUGW-FerH-ffLuc2-eGFP

   Plasmid

   #71393

   Purpose

   Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

   Depositor

   Glenn Merlino

   Article

   Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

   Insert

   FerH-ffLuc2-eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   FerH

   Available since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. ISceI-GR-RFP

   Plasmid

   #17654

   Depositor

   Tom Misteli

   Article

   Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

   Insert

   I-SceI-GRLBD (SCEI, Nr3c1 Rat, Budding Yeast)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   C655G mutation in the glucocorticoid receptor (se…

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. p8266 LentiCRISPR v2 hygro sgNT-1

   Plasmid

   #193977

   Purpose

   Expression of spCas9 and non-targeting control sgRNA

   Depositor

   Elizabeth White

   Article

   Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

   Insert

   spCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. hNET pcDNA3

   Plasmid

   #15475

   Depositor

   Randy Blakely

   Article

   Galli et al J Exp Biol. 1995 Oct . 198(Pt 10):2197-2

   Insert

   hNET (SLC6A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT3TS-NLS-TurboID

   Plasmid

   #163849

   Purpose

   In vitro transcription of nls-TurboID. Parton lab clone JST

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   TurboID
   

   Use

   In vitro transcription of rna

   Tags

   nls

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRMCD28

   Plasmid

   #167556

   Purpose

   Plasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mapping

   Depositor

   Renato Morona

   Article

   Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

   Type

   Empty backbone

   Use

   Tags

   PhoA'

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOpen-T5gene12/13

   Plasmid

   #165559

   Purpose

   Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T5 Exonuclease
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pJYS3_ΔcrtYf

   Plasmid

   #85542

   Purpose

   Constitutive transcription of FnCpf1 and crRNA of crtYf

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   crRNA of crtYf
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX303

   Plasmid

   #25897

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-C16orf80-flag

    Plasmid

    #70645

    Purpose

    Expresses C16orf80 in mammalian cells with FLAG-tag epitope.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    C16orf80 (CFAP20 Human)
    

    Use

    Retroviral

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-hSyn-EGFP (AAV5 trial size)

    Viral Prep

    #50465-AAV5.T

    Purpose

    Ready-to-use AAV5 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    EGFP

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

12. pTP943

    Plasmid

    #104157

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fab specific nanobody TP886
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVi U6_gRNA CMV_SadCas9-KRAB

    Plasmid

    #214609

    Purpose

    All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

    Depositor

    Ralf Gilsbach

    Article

    Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

    Inserts

    gRNA
    SadCas9
    

    Use

    AAV

    Tags

    NP NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CMV and U6

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFLAG/HA-MOV10

    Plasmid

    #10976

    Depositor

    Thomas Tuschl

    Article

    Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

    Insert

    MOV10 (MOV10 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMDC32-HPB

    Plasmid

    #32078

    Depositor

    Fumi Katagiri

    Article

    Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

    Type

    Empty backbone

    Use

    Gateway destination binary vector

    Tags

    HA-Prescission-Biotin

    Expression

    Mutation

    Promoter

    35S promoter

    Available since

    Dec. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDM067

    Plasmid

    #216820

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for C-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold2
    

    Use

    Tags

    VinnyLinker

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKIIa-hChR2(H134R)-EYFP

    Plasmid

    #26969

    Purpose

    AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

    Has Service

    AAV1, AAV2, AAV5, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Histidine 134 is mutated to Arginine

    Promoter

    CaMKIIa

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-4511NES

    Plasmid

    #138375

    Purpose

    Booster-ERK, a Red-Shifted Genetically Encoded FRET Biosensor for ERK activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-ERK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. BFP

    Plasmid

    #201383

    Purpose

    Control nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-EGFP-Cdc42-T17N

    Plasmid

    #12976

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 DN (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Promoter

    CMV

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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