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pGTMV
(Plasmid #118755)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 118755 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow Escherichia coli for 36 h at 37ºC in kanamycin plates. Grow Agrobacterium tumefaciens for 4 days at 28ºC in plates with rifampicin, tetracycline and kanamycin. Note that replication in A. tumefaciens requieres a pSoup-derivative as a helper plasmid.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tobacco mosaic virus
  • Alt name
    TMV
  • Species
    Tobacco mosaic virus
  • Promoter Cauliflower mosaic virus 35S

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGTMV was a gift from Jose-Antonio Daros (Addgene plasmid # 118755 ; http://n2t.net/addgene:118755 ; RRID:Addgene_118755)
  • For your References section:

    Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Shi X, Cordero T, Garrigues S, Marcos JF, Daròs JA, Coca M.. Plant Biotech Journal (2018) 10.1111/pbi.13038