AAVS1-DRGFP
(Plasmid #113193)

• Purpose: DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
• Depositing Lab: Adam Karpf
• Publication: CRISPR-mediated insertion of DR-GFP into the AAVS1 locus (unpublished)

Backbone

• Vector backbone: Hygro-Cas9 donor
• Backbone manufacturer: Danwei Huangfu
• Backbone size w/o insert (bp): 6079
• Total vector size (bp): 12553
• Modifications to backbone: Replaced Cas9 with a multiple cloning site then added DR-GFP cassette.
• Vector type: Mammalian Expression ; Donor construct with T2A-hygromycin for gene trapping.
• Selectable markers: Puromycin, Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DR-GFP
• Species: Synthetic; Two differentially mutated GFP genes oriented as direct repeats and separated by a puromycin selection marker
• Insert Size (bp): 6500

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MunI (not destroyed)
• 3′ cloning site: Mph1103 (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pAAVS1-T2A-Hygro-DR GFP.gb
• A portion of this plasmid was derived from a plasmid made by: The DR-GFP from subcloned from pDRGFP (Plasmid #26475).
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry