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pCDNA3-FLAG-Regnase-3_1-244 aa

Plasmid

#222659

Purpose

Mammalian expression vector to express 1-244 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-244 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated asparagine 245 to a stop codon

Promoter

CMV

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pScalps-Puro-FLAG-HA-Regnase-3 D252N

Plasmid

#222664

Purpose

Lentiviral vector to express mouse Regnase-3 D252N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

mutated aspartic acid 252 to asparagine to abroga…

Promoter

SFFV

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-FLAG-HA-Regnase-3 D252N

Plasmid

#222652

Purpose

For in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Mutation

mutated aspartic acid 252 to asparagine to abroga…

Promoter

T7

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141N

Plasmid

#222650

Purpose

For in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

T7

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-PES-H1-L92F-Q94Y

Plasmid

#218705

Purpose

Encodes codon optimized PES-H1 L92F-Q94Y enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

PES-H1

Tags

10xHis

Expression

Bacterial

Mutation

L92F-Q94Y

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_speG_Nterm_His

Plasmid

#220478

Purpose

heterologous expression for protein purification

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Tags

6xHs

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMCSG7-10xHis-ecRNHI*

Plasmid

#219523

Purpose

bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

Depositor

Arthur Palmer

Publication

Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

Insert

cysteine-free variant of the E. coli ribonuclease HI gene

Tags

N terminal 10xHis Tag

Expression

Bacterial

Mutation

Cysteine free variant

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-mRFP

Plasmid

#218709

Purpose

Encodes codon optimized mRFP enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

mRFP

Tags

10xHis

Expression

Bacterial

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-LCC-A2

Plasmid

#218691

Purpose

Encodes codon optimized LCC-A2 enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

LCC-A2

Tags

10xHis

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-LCC-ICCG-DAQI

Plasmid

#218689

Purpose

Encodes codon optimized LCC-ICCG DAQI enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

LCC-ICCG-DAQI

Tags

10xHis

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-PES-H1

Plasmid

#218704

Purpose

Encodes codon optimized PES-H1 enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

PES-H1

Tags

10xHis

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-TfCut2-L32E-S113E-T237Q

Plasmid

#218696

Purpose

Encodes codon optimized TfCut2 L32E-S113E-T237Q enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

TfCut2

Tags

10xHis

Expression

Bacterial

Mutation

L32E-S113E-T237Q

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-LCC-ICCG-RIP

Plasmid

#218688

Purpose

Encodes codon optimized LCC-ICCG RIP enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

LCC-ICCG-RIP

Tags

10xHis

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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3xMS2 TR knockin sgRNA 1

Plasmid

#207607

Purpose

sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Publication

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

cgactcgcccggcagcgcac

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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3xMS2 TR knockin sgRNA 2

Plasmid

#207608

Purpose

sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Publication

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

acccccaaacctgactgact

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-BFP-P2A-ppViperin

Plasmid

#217481

Purpose

Expression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Publication

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

BFP-P2A-otPKRFL

Expression

Mammalian

Promoter

CMV

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHIT348

Plasmid

#204515

Purpose

Bacterial expression of CEC-5 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen

Depositor

Yuji Kohara

Publication

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

C-terminal fragment of cec-5 (C. elegans)

Tags

6xHis, MBP

Expression

Bacterial

Promoter

T7lac

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-27bT

Plasmid

#203600

Purpose

Reporter plasmid carrying miR-27b target sequence

Depositor

Hiroyuki Mizuguchi

Publication

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

complementary sequence to miR27b-3p (MIR27B Human)

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-B4GALT3mut

Plasmid

#203602

Purpose

Reporter plasmid carrying B4GALT3 3'UTR with mutated miR27b target sequences.

Depositor

Hiroyuki Mizuguchi

Publication

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

beta-1,4-galactosyltransferase 3 (B4GALT3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Seed sequences in miR-27b target sequences were m…

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-MAIP1mut

Plasmid

#203604

Purpose

Reporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequences

Depositor

Hiroyuki Mizuguchi

Publication

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

matrix AAA peptidase interacting protein 1 (MAIP1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

miR-27b target sequences in the 3'UTR was mu…

Promoter

SV40

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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