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pVC2_C_03_fliR

Plasmid

#250054

Purpose

NT-CRISPR plasmid (pST_116) with gRNA targetting vps

Depositor

Georg Fritz

Article

Faber et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

NT-CRISPR plasmid (pST_116) with gRNA targetting vps

Use

Synthetic Biology

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pVC2_C_01_araA

Plasmid

#250052

Purpose

NT-CRISPR plasmid (pST_116) with gRNA targetting phaBAC

Depositor

Georg Fritz

Article

Faber et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

NT-CRISPR plasmid (pST_116) with gRNA targetting phaBAC

Use

Synthetic Biology

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pVC2_C_08_vps

Plasmid

#250059

Purpose

NT-CRISPR plasmid (pST_116) with gRNA targetting mScarlet

Depositor

Georg Fritz

Article

Faber et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

NT-CRISPR plasmid (pST_116) with gRNA targetting mScarlet

Use

Synthetic Biology

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-GeNL_SS

Plasmid

#244128

Purpose

Sensor scaffold luciferase derived from Oplophorus luc.

Depositor

Nathan Shaner

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Insert

GeNL_SS

Expression

Mammalian

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pTE5636

Plasmid

#250518

Purpose

Cell-free translocation reporter; translocation of a nanobody; N-terminal proOmpA-derived signal peptide with and extended hydrophobic core; C-terminal pep86 tag

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-p(extCore)OAss-nanobody-HiBiT

Tags

pep86

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTE5603

Plasmid

#250511

Purpose

Cell-free membrane insertion reporter; proteorhodopsin (UniProt ID: Q9F7P4; C-terminal transmembrane domain deleted); C-terminal pep104 tag

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-PR(-1tm)-pep104

Tags

pep104

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pJW53(uTEV3-DHFR2.0-IRES-mCherry)

Plasmid

#248363

Purpose

Lentiviral vector that can express destabilized uTEV3. Expressed off of CMV promoter with mCherry marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

uTEV3-DHFR2.0

Use

Lentiviral

Promoter

CMV

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Truncated_pvc10-Cas9

Plasmid

#243734

Purpose

PVC structural operon - untargeted (truncated) tail fiber (Pvc13) - spike tip (Pvc10) loaded with Cas9 on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Truncated_pvc10-Cas9

Expression

Bacterial

Available Since

Sept. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_pvc10-ZFD

Plasmid

#243743

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with C half of a zinc finger deaminase (ZFD) on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_pvc10-ZFD

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_Cas9-Pvc10

Plasmid

#243735

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with Cas9 on NTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_Cas9-Pvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_pvc8-Cas9

Plasmid

#243737

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike base (Pvc8) loaded with Cas9 on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_pvc8-Cas9

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDS_TempRecording

Plasmid

#241789

Purpose

pBBR1MCS replicative vector for Cas1-Cas2-RT and Spacer for transcriptional recording with a temperature sensor

Depositor

Marc Güell

Article

Nevot et al ACS Synth Biol. 2025 Jun 22. doi: 10.102

Insert

FBFP, Cas1-Cas2-RT_and CRISPR Array

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Ntom-Venus-ssrA

Plasmid

#223688

Purpose

PhoBIT1 component; Venus-tagged ssrA tag fused with Ntom for mitochondrial tethering

Depositor

Yubin Zhou

Article

Lee et al Nat Commun. 2025 Jul 28;16(1):6940. doi:

Insert

ssrA peptide

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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mCh-sspB(LOV2)

Plasmid

#223689

Purpose

PhoBIT1 component; mCherry-tagged sspB with LOV2 insertion

Depositor

Yubin Zhou

Article

Lee et al Nat Commun. 2025 Jul 28;16(1):6940. doi:

Insert

sspB(N)-LOV2-sspB(C)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pHK052.3

Plasmid

#235472

Purpose

Message phagemid carrying sgRNA3 (prom. J23119, backbone RSF1030)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA3

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAM358

Plasmid

#226138

Purpose

Expression of His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM296

Plasmid

#226132

Purpose

Expression of Ufd1L55R-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1L55R-3C-His6

Expression

Bacterial

Mutation

L55R

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM295

Plasmid

#226131

Purpose

Expression of Ufd1R64C-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1R64C-3C-His6

Expression

Bacterial

Mutation

R64C

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLPP4

Plasmid

#209966

Purpose

Contains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P40)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLP-EV

Plasmid

#209973

Purpose

Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

CDS (mock)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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