Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 11,951 results for: emb

Showing: 10101 - 10120 of 11951 results

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pNDGG008

Plasmid

#231312

Purpose

Vector part for BEVA; BEVA2.0 Position 4 sacB sucrose counterselection module, AmpR.

Depositor

Barney Geddes

Publication

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 4 sacB sucrose counterselection module, AmpR.

Expression

Bacterial

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGM65

Plasmid

#220170

Purpose

CRISRPi guide RNA against NACA

Depositor

Jonathan Weissman

Publication

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

NACA (sgRNA) (NACA Human)

Use

Lentiviral

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-StayGoldc4-Giantin (JDW 1355)

Plasmid

#229844

Purpose

A gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent protein

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

n2StayGoldc4

Use

Gateway Cloning

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-T-Lg

Plasmid

#216977

Purpose

Expression of GvpT-Linker-Large BiT

Depositor

George Lu

Publication

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpT-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-EF1a-pA (JDW 1222)

Plasmid

#224526

Purpose

A Gateway compatible 3' entry clone containing rat EF-1a polyA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF-1a polyA

Use

Gateway Cloning

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJB47

Plasmid

#226306

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with C to S CsoS2 MR1-5

Expression

Bacterial

Mutation

C292S, C310S, C351S, C369S, C467S, C485S, C526S, …

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pME-mCerulean3-TUBB1A (JDW 1367)

Plasmid

#224503

Purpose

Gateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cells

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean3-TUBB1A

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME H2B mCerulean (JDW 1150)

Plasmid

#224543

Purpose

A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mCerulean

Use

Gateway Cloning

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-N-Lg

Plasmid

#216963

Purpose

Expression of GvpN-Linker-Large BiT

Depositor

George Lu

Publication

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpN-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Sm-B

Plasmid

#216983

Purpose

Expression of Small BiT-Linker-GvpB

Depositor

George Lu

Publication

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Small BiT-Linker-GvpB

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-B-Sm

Plasmid

#216982

Purpose

Expression of GvpB-Linker-Small BiT

Depositor

George Lu

Publication

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpB-Linker-Small BiT

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(Q258K)

Plasmid

#224763

Purpose

In vitro transcription for Oocyte expression of hTRAAK(Q258K)

Depositor

Dan Minor

Publication

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Mutation

Q258K

Promoter

T7

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Lg-R

Plasmid

#216962

Purpose

Expression of Large BiT-Linker-GvpR

Depositor

George Lu

Publication

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpR

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-R-Lg

Plasmid

#216961

Purpose

Expression of GvpR-Linker-Large BiT

Depositor

George Lu

Publication

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpR-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(S118C)

Plasmid

#224762

Purpose

In vitro transcription for Oocyte expression of hTRAAK(S118C)

Depositor

Dan Minor

Publication

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Mutation

S118C

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Lg-F

Plasmid

#216966

Purpose

Expression of Large BiT-Linker-GvpF

Depositor

George Lu

Publication

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpF

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-F-Lg

Plasmid

#216965

Purpose

Expression of GvpF-Linker-Large BiT

Depositor

George Lu

Publication

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpF-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Lg-N

Plasmid

#216964

Purpose

Expression of Large BiT-Linker-GvpN

Depositor

George Lu

Publication

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpN

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Lg-S

Plasmid

#216972

Purpose

Expression of Large BiT-Linker-GvpS

Depositor

George Lu

Publication

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpS

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-S-Lg

Plasmid

#216971

Purpose

Expression of GvpS-Linker-Large BiT

Depositor

George Lu

Publication

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpS-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Showing: 10101 - 10120 of 11951 results

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