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pEPQD1CB0773

Plasmid

#177063

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

tryptophan decarboxylase (CrTDC) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0771

Plasmid

#177061

Purpose

MoClo Level 1, position 6, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

secologanin synthase (CrSLS1) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0118

Plasmid

#177050

Purpose

MoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid synthase (CrISY) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0775

Plasmid

#177065

Purpose

MoClo Level 1, position 2, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

strictosidine synthase (CrSTR) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0114

Plasmid

#177046

Purpose

MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol 8-oxidase (CrG8H) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0113

Plasmid

#177045

Purpose

MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol 8-oxidase (CrG8H) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0110

Plasmid

#177043

Purpose

MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol synthase (CrGES) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0910

Plasmid

#177033

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

loganic acid O-methyltransferase

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFPY2A

Plasmid

#165066

Purpose

Characterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFP

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>Y-FAST-ErB1.2A-yEGFP>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP104

Plasmid

#165059

Purpose

Integrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID9L

Plasmid

#165052

Purpose

Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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IDA3::NeonGreen exon2 (WS15)

Plasmid

#126876

Purpose

NeonGreen-tag embedded in the second exon of IDA3. Includes ampicilin and hygromycin resistance.

Depositor

Winfield Sale

Article

Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

Insert

IDA3

Use

Other

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK110

Plasmid

#110576

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP6 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK112

Plasmid

#110577

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP12b Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK113

Plasmid

#110578

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP32 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pOVC3

Plasmid

#131173

Purpose

Cloning vector containing B-A-C casette

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pC0.165

Plasmid

#119634

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS1 Up Flank

Use

Synthetic Biology

Mutation

1 nucleotide insertion between nt 21 and 22 in NS…

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC2

Plasmid

#131172

Purpose

Cloning vector containing A-C-B casette

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1

Plasmid

#131171

Purpose

Cloning vector containing A-B-C casette

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.007

Plasmid

#119671

Purpose

Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Pcpc560-Dx2

Use

Synthetic Biology

Mutation

bp 89 T to C, bp 187 C to A, Duplicated downstrea…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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