We narrowed to 4,697 results for: pcr

1. STAgR_Neo

   Plasmid

   #102992

   Purpose

   Can be used as backbone for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Type

   Empty backbone

   Use

   CRISPR; Pcr template for stagr vectors

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHyg-yeGFP-His6

   Plasmid

   #212781

   Purpose

   C-terminal PCR-based tagging with yeGFP-His6 in budding yeast with HygR selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-kanMX6-P3nmt1

   Plasmid

   #39280

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus

   Promoter

   Available since

   Aug. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-His3MX6-PGAL1-GFP

   Plasmid

   #41616

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-kanMX6-PGAL1-3HA

   Plasmid

   #41608

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, A. gossypii translation elongation factor 1…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRB14

   Plasmid

   #52522

   Purpose

   expresses a myc-tagged version of hCas9 in Drosophila

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   S. pyogenes cas9 with humanized codon bias
   

   Use

   Tags

   myc

   Expression

   Insect

   Mutation

   Promoter

   tubulin

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. STAgR_tdTomato

   Plasmid

   #102993

   Purpose

   Can be used as backbone for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Type

   Empty backbone

   Use

   CRISPR; Pcr template for stagr vectors

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-kanMX6-PGAL1-GST

   Plasmid

   #41611

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA6a-kanMX6-PGAL1-GFP

   Plasmid

   #41614

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCaUra3-CUbo-AID*-GFP

    Plasmid

    #212775

    Purpose

    C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-His3MX6-PGAL1-3HA

    Plasmid

    #41610

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pANY1

    Plasmid

    #110949

    Purpose

    Universal mini-vector for efficient cloning of DNA and expression of protein in E. coli.

    Depositor

    Yingfeng An

    Article

    Liu et al Biochem Biophys Res Commun. 2018 Mar 18;

    Insert

    ccd B cassette
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHyg-CUbo(K48R/K63R)-His6

    Plasmid

    #212780

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R/K63R)-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHyg-CUbo(K48R)-9myc

    Plasmid

    #212787

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R)-9myc in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHyg-CUbo(K63R)-yeGFP

    Plasmid

    #212768

    Purpose

    C-terminal PCR-based tagging with CUbo(K63R)-yeGFP in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHyg-CUbo(K48R/K63R)-yeGFP

    Plasmid

    #212769

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHyg-CUbo-yeGFP

    Plasmid

    #212766

    Purpose

    C-terminal PCR-based tagging with CUbo-yeGFP in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHyg-CUbo(K48R)-yeGFP

    Plasmid

    #212767

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCaUra3-CUbo-yeGFP

    Plasmid

    #212771

    Purpose

    C-terminal PCR-based tagging with CUbo-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCaUra3-CUbo(K48R)-yeGFP

    Plasmid

    #212772

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge