We narrowed to 1,086 results for: gibson assembly

1. pKG3658_pPV2-NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

   Plasmid

   #239699

   Purpose

   BsaI Golden Gate assembly plasmid encoding the COMET NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720 CDS

   Depositor

   Kate Galloway

   Article

   Peterman et al Nucleic Acids Research, Volume 53, Issue

   Insert

   NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720
   

   Use

   Synthetic Biology

   Available Since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.minBG-EGFP-W3SL_BbsI(GGA)

   Plasmid

   #231362

   Purpose

   minBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   EGFP
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

   Plasmid

   #231363

   Purpose

   Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   EGFP
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. KTK_095

   Plasmid

   #180524

   Purpose

   dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBICI_high ori

   Plasmid

   #179273

   Purpose

   Expresses CuCBP and BaSP in E. coli BL21(DE3)

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Promoter

   T7 lacO

   Available Since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDUBI

   Plasmid

   #179274

   Purpose

   Expresses CcCDP monocistronically and CuCBP and BaSP bicistronically

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

   Inserts

   cellodextrin phosphorylase
   cellobiose phosphorylase
   C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
   

   Use

   Synthetic Biology

   Tags

   6xHIS and Strep-Tag II

   Expression

   Bacterial

   Mutation

   sucrose phosphorylase

   Promoter

   T7 lacO

   Available Since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Halo-Rab6A

   Plasmid

   #190171

   Purpose

   Expresses HaloTag-Rab6A in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   HaloTag-Rab6A
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEND-351_Cacnes_dCas9_CRISPRi

   Plasmid

   #225617

   Purpose

   Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR

   Plasmid

   #229995

   Purpose

   PiggyBac sgRNA cloning plasmid with tdTomato reporter with a capture sequence (cs1)

   Depositor

   Christian Mayer

   Article

   Dvoretskova et al Nat Neurosci. 2024 May;27(5):862-872. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   NotI site for NEBuilder HiFi DNA Assembly with ss…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMMK ENTR 2 hU6 ACTB sgRNA

    Plasmid

    #206270

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNA

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    ACTB sgRNA
    

    Use

    CRISPR; Multimate/gateway entr 2

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMMK ENTR 4 CMV eGFP

    Plasmid

    #206272

    Purpose

    ENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoter

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    eGFP
    

    Use

    Multimate/gateway entr 4

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMMK ENTR 2 hU6 HEK3 PegRNA

    Plasmid

    #206277

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNA

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    HEK3 PegRNA
    

    Use

    CRISPR; Multimate/gateway entr 2

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMMK ENTR 3 polH aeBlue polH VSV-G

    Plasmid

    #206278

    Purpose

    ENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    aeBlue, VSV-G
    

    Use

    Multimate/gateway entr 3

    Expression

    Bacterial and Insect

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMMK ENTR 4 CMV mTagBFP

    Plasmid

    #206279

    Purpose

    ENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mTagBFP
    

    Use

    Multimate/gateway entr 4

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGG431

    Plasmid

    #165615

    Purpose

    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Corrected homology relative to wild type SpCas9 (…

    Promoter

    TEF1

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MBP-GFP-RGG

    Plasmid

    #242453

    Purpose

    Expresses the MBP-GFP-RGG fusion protein.

    Depositor

    Benjamin Schuster

    Article

    Kelley et al Proc Natl Acad Sci U S A. 2021 Dec 21;11

    Insert

    MBP-GFP-RGG
    

    Tags

    His and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-GFP-RGG

    Plasmid

    #242454

    Purpose

    Expresses the GST-GFP-RGG fusion protein.

    Depositor

    Benjamin Schuster

    Article

    Kelley et al Proc Natl Acad Sci U S A. 2021 Dec 21;11

    Insert

    GST-GFP-RGG
    

    Tags

    GST and His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpyTag-sfGFP

    Plasmid

    #184226

    Purpose

    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpyTag-sfGFP
    

    Tags

    His6, SpyTag, and TEV protease cleavage site

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOD_009

    Plasmid

    #159520

    Purpose

    Rifampicin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pJBJZ_003

    Plasmid

    #155358

    Purpose

    Rifampicin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge