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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,121 results for: gibson assembly

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Showing: 1041 - 1060 of 1121 results

  1. pNC-Y

    Plasmid
    #238998
    Purpose
    Node C carrying sgRNA-1 that can be used for the assembly of pEND-11Y or pEND-ZY
    Depositor
    Javier Santos-Moreno
    Publication
    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2
    Insert
    sgRNA-1 downstream of a weak constitutive promoter and a binding site for sgRNA-2
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    WT
    Available since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    238998-plasmid-map-sequence-id-479360

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  13. pNB-Rep

    Plasmid
    #238996
    Purpose
    Node B carrying the sfGPF reporter that can be used for the assembly of pEND-11Y, pEND-Y11, pEND-Z11 or pEND-ZY
    Depositor
    Javier Santos-Moreno
    Publication
    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2
    Insert
    sfGFP-MarAn10 downstream of a strong constitutive promoter and a binding site for sgRNA-1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    WT
    Available since
    Dec. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    238996-plasmid-map-sequence-id-495027

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  15. MIOX only

    Plasmid
    #166681
    Purpose
    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Publication
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    MIOX (Miox Mouse, Budding Yeast, Synthetic)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    166681-plasmid-map-sequence-id-329928

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  16. pTEF1-UDH

    Plasmid
    #166679
    Purpose
    Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Claudia Vickers
    Publication
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    UDH
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    TEF1
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166679-plasmid-map-sequence-id-329863

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  20. MZB164

    Plasmid
    #217842
    Purpose
    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Publication
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    HaloTag
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217842-plasmid-map-sequence-id-433000

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Showing: 1041 - 1060 of 1121 results