We narrowed to 18,276 results for: multi
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Plasmid#122243PurposeExpresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backboneDepositorUseAdenoviralTagsSignal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…ExpressionMammalianMutationdeleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…PromoterCMVAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only
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pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry
Plasmid#170183PurposeExpression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptideDepositorInsertsgBFP
ExpressionMammalianPromoterhU6Available SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC011
Plasmid#171143PurposeExpression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmidDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(T17N)
Plasmid#164902PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domainDepositorInsertRaichuEV-Rac1 (RAC1 Human)
ExpressionMammalianMutationT17N mutation in the Rac1 domain which make the s…Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir181b-1-CMV2-mir1
Plasmid#164670PurposeExpress miR-181b-1 and miR-1-1 in mammalian cellsDepositorAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir125-CMV2-mir1
Plasmid#164668PurposeExpress miR-125a and miR-1-1 in mammalian cellsDepositorAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPK-351
Plasmid#157921PurposepcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBDDepositorInsertsPIF3-MTAD
IRES
PhyB(1-621)-SV40NLS-Gal4DBD
UseSynthetic Biology; OptogeneticsTagsHA tag and SV40NLSExpressionMammalianMutationpif3 1-523PromoterCMVAvailable SinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT[Hygro]_GBP-3xFlag-DEST
Plasmid#157813PurposeA gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selectionDepositorInsertGFP-binding protein
UseFor drosophila cellsTags3xFlagExpressionInsectPromotermetallothioneinAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_T5
Plasmid#155175Purposesee pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-800)-Strep
Plasmid#141344PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-800)-Strep
TagsGB1-His6-TEV and Strep-tag (WSHPQFEK)ExpressionBacterialAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-Kv4.2 K+ channel [L28/4R]
Plasmid#149453PurposeMammalian Expression Plasmid of anti-Kv4.2 K+ channel (Rat). Derived from hybridoma L28/4.DepositorInsertanti-Kv4.2 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnd2 Mouse)
ExpressionMammalianPromoterDual CMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-Snapin/SNAPAP [L8/15R]
Plasmid#140071PurposeMammalian Expression Plasmid of anti-Snapin/SNAPAP (Human). Derived from hybridoma L8/15.DepositorInsertanti-Snapin/SNAPAP (Human) recombinant mouse monoclonal antibody (SNAPIN Mouse)
ExpressionMammalianPromoterdual CMVAvailable SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL90-Nat-2xHO
Plasmid#139069PurposeCRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selectionDepositorInsertTwo guide RNAs targeting the HO endonuclease
ExpressionBacterial and YeastPromoterTDH3Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK354
Plasmid#129698PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120010799
ExpressionBacterialAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
K51/1RAnti-SH3GL1/Endophilin A2 [K51/1R]
Plasmid#128621PurposeMammalian Expression Plasmid of anti-SH3GL1/Endophilin A2 (Rat). Derived from hybridoma K51/1.DepositorInsertanti-SH3GL1/Endophilin A2 (Rat) recombinant mouse monoclonal antibody (Sh3gl1 Mouse)
ExpressionMammalianPromoterDual CMVAvailable SinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-Pan-Kvbeta K+ channel [K25/73R]
Plasmid#128617PurposeMammalian Expression Plasmid of anti-Pan-Kvbeta K+ channel. Derived from hybridoma K25/73.DepositorInsertanti-Pan-Kvbeta K+ Channel Pan recombinant mouse monoclonal antibody
ExpressionMammalianPromoterDual CMVAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEcRNAP5
Plasmid#128939PurposepET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, ZDepositorInsertsTagsHisExpressionBacterialMutationResidues 234-241 are substituted for a PreScissio…PromoterT7Available SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
PacrAB-acrAB-rfp + PmutS-mutS-yfp
Plasmid#121443Purposeplasmid expressing translational fusions of AcrAB and MutSDepositorInsertsPmutS-mutS-yfp
PacrAB-acrAB-rfp
UseSynthetic Biology; Pbb vectorExpressionBacterialPromoterPacrAB and PmutSAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-Flrt3 [N353/13R]
Plasmid#114486PurposeMammalian Expression Plasmid of anti-Flrt3 (Mouse). Derived from hybridoma N353/13.DepositorInsertanti-Flrt3 (Mus musculus) recombinant mouse monoclonal antibody (Flrt3 Mouse)
ExpressionMammalianPromoterdual CMVAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113687PurposeSaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.DepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSPromoterCytomegalo Virus(CMV)Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only