|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||103101||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerNEB(New England Biolabs)
Vector typeCRISPR ; Cloning vector
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameE6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
Insert Size (bp)3450
- Cloning method Gibson Cloning
- 5′ sequencing primer gctgcaaggcgattaagttg
- 3′ sequencing primer cggctcgtatgttgtgtgg (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC19-E6I4Q5 was a gift from Duhee Bang (Addgene plasmid # 103101 ; http://n2t.net/addgene:103101 ; RRID:Addgene_103101)
For your References section:High-throughput construction of multiple cas9 gene variants via assembly of high-depth tiled and sequence-verified oligonucleotides. Cho N, Seo HN, Ryu T, Kwon E, Huh S, Noh J, Yeom H, Hwang B, Ha H, Lee JH, Kwon S, Bang D.. Nucleic Acids Research (2018) gky112 10.1093/nar/gky112