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pLEW100v5-PUR-VSG-228.100bp_offset

Plasmid

#245115

Purpose

Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer overlapping AnTat1.1 position 694, centered around common VSG-228 insertion

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228:100_offset

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-HNRNPL_sgRNA_3

Plasmid

#242892

Purpose

PiggyBac cargo vector with HNRNPL sgRNA 3 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Murvin et al bioRxiv 2025.07.16.665154

Insert

HNRNPL (Hnrnpl Mouse)

Use

CRISPR

Promoter

U6

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLX304-mito-Lantern

Plasmid

#250920

Purpose

Expresses Lantern fused to the N-terminal 23 amino acid residues of human COX4 in mammalian cells

Depositor

Peng Zou

Article

Fang et al Research Square 2025 [Preprint]. https:/

Insert

mito-Lantern

Use

Lentiviral

Tags

COX4 mitochondrial targeting sequence, V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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E. coli Beta sliding clamp (dnaN) in pet28PP

Plasmid

#217412

Purpose

Expresses E. coli Beta sliding clamp with a His-tag in bacterial cells

Depositor

Brian Kelch

Article

Landeck et al J Biol Chem. 2024 Apr;300(4):107166. doi

Insert

Beta sliding clamp (dnaN E. coli)

Tags

His

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-nCas9-PolI5M

Plasmid

#249066

Purpose

Expresses nucleus-localized nCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

nCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI5M

Plasmid

#249060

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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p-mCherry-dCas9-PolI5M

Plasmid

#249065

Purpose

Expresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

dCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p-mCherry-enCas9(D10A)

Plasmid

#249063

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Neo1 gRNA

Plasmid

#246575

Purpose

CRISPR vector co-expressing Cas9 and a mouse Neo1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Neo1 gRNA (Neo1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Nrp1 gRNA

Plasmid

#246578

Purpose

CRISPR vector co-expressing Cas9 and a mouse Nrp1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Nrp1 gRNA (Nrp1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Plxna1 gRNA

Plasmid

#246574

Purpose

CRISPR vector co-expressing Cas9 and a mouse Plxna1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Plxna1 gRNA (Plxna1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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huADF S3A

Plasmid

#245943

Purpose

Expressing a constitutively active human ADF

Depositor

James Bamburg

Article

Agnew et al J Biol Chem. 1995 Jul 21;270(29):17582-7

Insert

actin depolymerizing factor (ADF) (DSTN Human)

Expression

Mammalian

Mutation

S3A

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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TtEnd_E448A_pEHISTEV

Plasmid

#179733

Purpose

TtEnd_E448A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which Glu448 has been mutated to Ala448.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

Glu448 has been mutated to Ala448.

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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TtEnd_E329A_pEHISTEV

Plasmid

#179734

Purpose

TtEnd_E329A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which Glu329 has been mutated with Ala329

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

Glu329 has been mutated to Ala329.

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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TtEnd_H280A_pEHISTEV

Plasmid

#179735

Purpose

TtEnd_H280A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which His280 has been mutated with Ala280.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

His280 has been mutated to Ala280.

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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TtEnd_CDC_E329A

Plasmid

#179737

Purpose

TtEnd_CDC_E329A expresses TtEnd_CDC_pEHISTEV, in which Glu329 has been mutated with Ala329.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

Glu329 has been mutated with Ala329

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pWPI_GW_BioID2_T2A_Blast

Plasmid

#214831

Purpose

Destination vector for cloning BioID2 fusion proteins

Depositor

Hani Goodarzi

Article

Khoroshkin et al Nat Commun. 2024 Sep 9;15(1):7872. doi:

Type

Empty backbone

Use

Lentiviral

Tags

BioID2

Expression

Bacterial and Mammalian

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3583)

Plasmid

#239260

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3584)

Plasmid

#239261

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-GalK-USP-ET64

Plasmid

#234662

Purpose

Expression an ELP-tagged fusion enzyme of GalK and AtUSP

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

galactokinase
UDP-sugar pyrophosphorylase (USP Mustard Weed)

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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