We narrowed to 1,086 results for: gibson assembly

1. pJBJZ_001

   Plasmid

   #155357

   Purpose

   Gentamycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

   Depositor

   Elisabeth Raleigh

   Article

   Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pJBJZ_006

   Plasmid

   #155359

   Purpose

   Zeomycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

   Depositor

   Elisabeth Raleigh

   Article

   Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. SspBmicro-mCh-p60

   Plasmid

   #190168

   Purpose

   Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   SspBmicro-mCherry-p60
   

   Tags

   SspBmicro-mCherry

   Expression

   Mammalian

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyCatcher003-mi3

   Plasmid

   #159995

   Purpose

   Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyCatcher003-mi3
   

   Tags

   C-Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PsaA

   Plasmid

   #159997

   Purpose

   Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   PsaA (19-309)
   

   Tags

   His6-tag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Spy0469

   Plasmid

   #159996

   Purpose

   Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   Spy0469 (24-389)
   

   Tags

   His6-tag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOD_003

   Plasmid

   #155362

   Purpose

   Zeomycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

   Depositor

   Elisabeth Raleigh

   Article

   Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pSIN-TRE-SspB-mCh-p60

   Plasmid

   #190170

   Purpose

   Doxycycline-inducible lentivirus vector to express SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   SspBmicro-mCherry-p60
   

   Use

   Lentiviral

   Tags

   SspBmicro-mCherry

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AviTag-SpyTag003-MBP

   Plasmid

   #184227

   Purpose

   Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   AviTag-SpyTag003-MBP
   

   Tags

   AviTag, His6, SpyTag003, and Thrombin cleavage si…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-yomRuby3-SpHIS5

    Plasmid

    #204287

    Purpose

    For C-terminal tagging using yeast optimized mRuby3

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pFA6-yomRuby3-SpHIS5
    

    Tags

    yomRuby3

    Expression

    Yeast

    Promoter

    none

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWZ699

    Plasmid

    #208117

    Purpose

    Payload yeast assembly vector with TK-mActb on backbone

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    Synthetic Biology; Copy number inducible

    Expression

    Bacterial, Mammalian, and Y…

    Promoter

    Human PGK1 for thymidine kinase

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMMK ENTR 2 CMV mKO2-hCdt1

    Plasmid

    #206282

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly. Encodes mKO2-hCdt1 under the control of CMV

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mKO2-hCdt1 (CDT1 Human)
    

    Use

    Multimate/gateway entr 2

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMMK ENTR 1 HITI-2c ACTB

    Plasmid

    #206269

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly. Encodes HITI-2c donor for ACTB C-terminal tagging (mCherry T2A Puro) in human cells

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    HITI-2c donor
    

    Use

    CRISPR; Multimate/gateway entr 1

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGG442

    Plasmid

    #165616

    Purpose

    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    

    Use

    CRISPR

    Tags

    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

    Expression

    Yeast

    Mutation

    Corrected homology relative to wild type SpCas9 (…

    Promoter

    TEF1

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. EB3N-VVDfast-mCl3-iLID

    Plasmid

    #190165

    Purpose

    Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    EB3N-VVDfast-mClover3-iLID
    

    Tags

    -VVDfast-mClover3-iLID

    Expression

    Mammalian

    Mutation

    1-189 aa from EB3

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. EB3N-VVDfast-Halo-iLID

    Plasmid

    #190166

    Purpose

    Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    EB3N-VVDfast-Halo-iLID
    

    Tags

    -VVDfast-mClover3-iLID

    Expression

    Mammalian

    Mutation

    1-189 aa from EB3

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. iLID-mCl3-MAP7N

    Plasmid

    #190167

    Purpose

    Expresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    iLID-mClover3-MAP7N
    

    Tags

    iLID-mClover3

    Expression

    Mammalian

    Mutation

    aa 1-309 from human MAP7 delta175-213

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. NDV-HN-SpyTag

    Plasmid

    #160000

    Purpose

    Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    NDV-HN-SpyTag
    

    Tags

    His6-tag

    Expression

    Mammalian

    Promoter

    Chicken beta-actin promoter

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpyTag003-OspC

    Plasmid

    #159998

    Purpose

    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyTag003-OspC (20-209) C129S
    

    Tags

    His6-tag and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    C129S mutation improves expression level.

    Promoter

    T7

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6P1 UBXN11

    Plasmid

    #169017

    Purpose

    Expresses GST-UBXN11 (human) in bacteria

    Depositor

    Hemmo Meyer

    Article

    Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

    Insert

    UBXN11 (UBXN11 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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