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We narrowed to 1,256 results for: gibson assembly

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Showing: 1061 - 1080 of 1256 results

  1. pUC19-B5JU35

    Plasmid
    #103087
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B5JU35(Cas9 coding gene from gamma proteobacterium HTCC5015)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103087-plasmid-map-sequence-id-201737

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  2. pUC19-B5XLC1

    Plasmid
    #103088
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B5XLC1(Cas9 coding gene from Streptococcus pyogenes serotype M49 (strain NZ131))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103088-plasmid-map-sequence-id-201738

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  3. pUC19-C4ZA16

    Plasmid
    #103090
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    C4ZA16(Cas9 coding gene from Eubacterium rectale (strain ATCC 33656 / VPI 0990))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103090-plasmid-map-sequence-id-201740

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  9. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

    Plasmid
    #132927
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Insert
    humanized SpCas9
    Use
    CRISPR and Synthetic Biology; Pmagic gateway entr…
    Available Since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    132927-plasmid-map-sequence-id-314451

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  10. pHRSV_CHARMS

    Plasmid
    #233920
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233920-plasmid-map-sequence-id-469222

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  11. pDONOR 6colors

    Plasmid
    #229974
    Purpose
    bigMamAct assembled plasmid for 6colors cellular labelling
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Insert
    mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    See Depositor Comments
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229974-plasmid-map-sequence-id-461259

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  12. pMMK ENTR 4 CMV GST mTagBFP

    Plasmid
    #206259
    Purpose
    ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    GTS mTagBFP
    Use
    Multimate/gateway entr 4
    Tags
    BFGT1 (Golgi localisation peptide)
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206259-plasmid-map-sequence-id-406393

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  13. pMMACE DEST CMV H2B iRFP

    Plasmid
    #206253
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    H2B iRFP713
    Use
    Recombinant baculovirus production, multimate/gat…
    Tags
    iRFP713
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206253-plasmid-map-sequence-id-406403

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  14. pMMACE DEST CMV SpCas9

    Plasmid
    #206268
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9
    Use
    CRISPR; Recombinant baculovirus production
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206268-plasmid-map-sequence-id-406404

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  16. pMMACE DEST CMV PE2

    Plasmid
    #206276
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    PE2
    Use
    CRISPR; Recombinant baculovirus production, multi…
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206276-plasmid-map-sequence-id-406406

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  17. pUC19-Q03JI6

    Plasmid
    #103123
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q03JI6 (Cas9 coding gene from Campylobacter jejuni)
    Use
    CRISPR; Cloning vector
    Tags
    SV40NLS
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103123-plasmid-map-sequence-id-201885

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  18. PMXS-SLC1A3

    Plasmid
    #72873
    Purpose
    The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    SLC1A3 (SLC1A3 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pCMU-4

    Plasmid
    #172353
    Purpose
    CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA
    Depositor
    Eriko Takano
    Article
    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(
    Insert
    D4-TheoR-cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    D4
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172353-plasmid-map-sequence-id-350007

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Showing: 1061 - 1080 of 1256 results