Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus.
Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, Welsh GI, Berger I
Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587. PubMed Article
Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, Welsh GI, Berger I
Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206252 | MultiMate-Rainbow | Expression of 7 fluorescently labelled proteins in mammalian cells. Can be used to generate recombinant baculovirus particles. |
| 206253 | pMMACE DEST CMV H2B iRFP | DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system. |
| 206254 | pMMK ENTR 1 CMV EYFP Tubulin | ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes EYFP Tubulin under the control of CMV promoter. |
| 206255 | pMMK ENTR 2 CMV mTFP1 Actin | ENTR Vector 2 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTFP1 Actin under the control of CMV promoter. |
| 206256 | pMMK ENTR 3 CMV mito mCherry | ENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter. |
| 206258 | pMMK ENTR 1 CMV mAG beta-catenin | ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter. |
| 206259 | pMMK ENTR 4 CMV GST mTagBFP | ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter. |
| 206260 | pMMK ENTR 1 | ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone. |
| 206261 | pMMK ENTR 2 | ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone. |
| 206262 | pMMK ENTR 3 | ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone. |
| 206263 | pMMK ENTR 4 | ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone. |
| 206264 | pMMDS DEST | DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system. |
| 206265 | pMMDS 2 colors | Expression of 2 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system) |
| 206266 | MultiMate 5 colors | Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system) |
| 206267 | MultiMate-HITI-2c ACTB | All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus. |
| 206268 | pMMACE DEST CMV SpCas9 | DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system. |
| 206269 | pMMK ENTR 1 HITI-2c ACTB | ENTR Vector 1 for MultiSite Gateway assembly. Encodes HITI-2c donor for ACTB C-terminal tagging (mCherry T2A Puro) in human cells |
| 206270 | pMMK ENTR 2 hU6 ACTB sgRNA | ENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNA |
| 206272 | pMMK ENTR 4 CMV eGFP | ENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoter |
| 206273 | MultiMate-PE3 HEK3 | Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing. |
| 206274 | MultiMate-PE2 HEK3 | Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system. |
| 206276 | pMMACE DEST CMV PE2 | DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system. |
| 206277 | pMMK ENTR 2 hU6 HEK3 PegRNA | ENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNA |
| 206278 | pMMK ENTR 3 polH aeBlue polH VSV-G | ENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters. |
| 206279 | pMMK ENTR 4 CMV mTagBFP | ENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter. |
| 206280 | MultiMate-Cell-cycle | Expression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles. |
| 206281 | pMMK ENTR 1 CMV mAG h-Geminin | ENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMV |
| 206282 | pMMK ENTR 2 CMV mKO2-hCdt1 | ENTR Vector 2 for MultiSite Gateway assembly. Encodes mKO2-hCdt1 under the control of CMV |