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Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus.

Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, Welsh GI, Berger I
Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
206252MultiMate-RainbowExpression of 7 fluorescently labelled proteins in mammalian cells. Can be used to generate recombinant baculovirus particles.
206253pMMACE DEST CMV H2B iRFPDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
206254pMMK ENTR 1 CMV EYFP TubulinENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes EYFP Tubulin under the control of CMV promoter.
206255pMMK ENTR 2 CMV mTFP1 ActinENTR Vector 2 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTFP1 Actin under the control of CMV promoter.
206256pMMK ENTR 3 CMV mito mCherryENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.
206258pMMK ENTR 1 CMV mAG beta-cateninENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter.
206259pMMK ENTR 4 CMV GST mTagBFPENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.
206260pMMK ENTR 1ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.
206261pMMK ENTR 2ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.
206262pMMK ENTR 3ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.
206263pMMK ENTR 4ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.
206264pMMDS DESTDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.
206265pMMDS 2 colorsExpression of 2 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)
206266MultiMate 5 colorsExpression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)
206267MultiMate-HITI-2c ACTBAll in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.
206268pMMACE DEST CMV SpCas9DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
206269pMMK ENTR 1 HITI-2c ACTBENTR Vector 1 for MultiSite Gateway assembly. Encodes HITI-2c donor for ACTB C-terminal tagging (mCherry T2A Puro) in human cells
206270pMMK ENTR 2 hU6 ACTB sgRNAENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNA
206272pMMK ENTR 4 CMV eGFPENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoter
206273MultiMate-PE3 HEK3Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing.
206274MultiMate-PE2 HEK3Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system.
206276pMMACE DEST CMV PE2DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
206277pMMK ENTR 2 hU6 HEK3 PegRNAENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNA
206278pMMK ENTR 3 polH aeBlue polH VSV-GENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.
206279pMMK ENTR 4 CMV mTagBFPENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.
206280MultiMate-Cell-cycleExpression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles.
206281pMMK ENTR 1 CMV mAG h-GemininENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMV
206282pMMK ENTR 2 CMV mKO2-hCdt1ENTR Vector 2 for MultiSite Gateway assembly. Encodes mKO2-hCdt1 under the control of CMV

Antibodies from Article