MultiMate-PE3 HEK3
(Plasmid #206273)

• Purpose: Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing.
• Depositing Lab: Imre Berger
• Publication: Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587.

Backbone

• Vector backbone: pMMACE DEST CMV PE2
• Vector type: Mammalian Expression, CRISPR ; Recombinant baculovirus production

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Gentamicin, 25 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
• Species: Other

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • MultiMate-PE3 HEK3.gb
• A portion of this plasmid was derived from a plasmid made by: PE2 was obtained by restriction digestion from pCMV PE2 (Addgene plasmid #132775, Anzalone et al. Nature 2019) and the HEK3 PegRNA was PCR amplified from pU6-Sp-pegRNA-HEK3_CTT_ins (Addgene plasmid #132778, Anzalone et al. Nature 2019 ). HEK3 nicking sgRNA (Anzalone et al. Nature 2019) was synthesised Twist Bioscience. aeBlue (Liljeruhm J. et al J. Biol. Eng. 2018) was synthesised by Twist Bioscience and VSVG-G was amplified from pMD2.G (Didier Trono lab, Addgene plasmid # 12259)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry