MultiMate 5 colors
(Plasmid #206266)

• Purpose: Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)
• Depositing Lab: Imre Berger
• Publication: Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 10.1093/nar/gkac587.

Backbone

• Vector backbone: pMMACE DEST CMV H2B IRFP713
• Vector type: Mammalian Expression ; Recombinant baculovirus production, CRE Acceptor (MultiBac)

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
• Species: Other

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • MultiMate 5 colors.gb
• A portion of this plasmid was derived from a plasmid made by: H2B-iRFP was PCR amplified from pCAG-H2BtdiRFP-IP (Addgene plasmid #47884, Miyanari Y. et al. Nat Struct Mol Biol. 2013)EYFP-Tubulin and mTFP1-Actin were amplified from 5-colours MultiBac vectors (Mansouri et al. Nat Comm 2016)Mito-mCherry was generated by fusing mCherry from 7TGC (Addgene plasmid#24304) to the COX8 mitochondrial targeting sequence from 5-colours MultiBac vectors (Mansouri et al. 2016)CyOFP1 (Chu et al. Nat Biotechnology 2016)fused to the endoplasmic reticulum targeting sequence (ER) was synthesised by Twist Bioscience

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry