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Bxbi+PhiC31 GFP Bandpass BAC Reporter

Plasmid

#78218

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available Since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

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Bxbi+TP901 GFP Bandpass BAC Reporter

Plasmid

#78229

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2-sfGFP

Plasmid

#88920

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-YFP-HA no-pA

Plasmid

#82590

Purpose

3' entry vector containing HA epitope fused to C-terminus of YFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

YFP-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-HA no-pA

Plasmid

#82592

Purpose

3' entry vector with HA epitope fused to C-terminus of mCherry without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mCherry-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3d-FL3

Plasmid

#78379

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFlag
ura4+ (ura4 Fission Yeast)

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3d-HA3

Plasmid

#78380

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
ura4+ (ura4 Fission Yeast)

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pProm1_BCD20-GFP

Plasmid

#80651

Purpose

Promoter1 with BCD20-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pProm1_BCD21-GFP

Plasmid

#80652

Purpose

Promoter1 with BCD21-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pTC151

Plasmid

#70015

Purpose

TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a non-replicating, mutant version of the Gemini Viral Replicon

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (TALENs) + Donor + GVR

Use

TALEN

Expression

Plant

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMZ030

Plasmid

#66970

Purpose

Expresses wild type zebrafish Chk1 with C-term GFP fluorescent tag

Depositor

Michael Lampson

Article

Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

Insert

wildtype zebrafish Chk1

Tags

GFP

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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3x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64391

Purpose

encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Expression

Yeast

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
LIIc F 1-2 RNAi

Plasmid

#54402

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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LII dy 1-3

Plasmid

#54374

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

dummy 1-3

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LII dy 4-5

Plasmid

#54376

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

dummy 4-5

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LII dy 2-3

Plasmid

#54375

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

dummy 2-3

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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PM-ST1!TA

Plasmid

#48653

Purpose

Bacterial ST1 crRNA expression: targets ST1 to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial ST1 crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pTW2657

Plasmid

#252577

Purpose

TRV2 multiplex vector expressing Ymu1-WFR, AtPDS3 gRNA12 and AtCHLl1 gRNA4

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1-WFR with AtPDS3 gRNA 12 and AtCHLl1 gRNA4

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pGY214

Plasmid

#239805

Purpose

Express EGFP and mRFP1 simultaneously in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

EGFP
mRFP1

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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