167,564 results

1. pIG-804_HA-GD2-28z_CAR_BATF-TFAP4

   Plasmid

   #207491

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   BATF-TFAP4, HA-GD2-28z_CAR (BATF Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCas9-BFP

   Plasmid

   #78545

   Purpose

   Fusion protein Cas9+BFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Fusion protein with Cas9

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AiP13897 - pAAV-AiE0671m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3897)

   Plasmid

   #224038

   Purpose

   AiE0671m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

   Depositor

   Jonathan Ting, Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   NA

   Promoter

   minBG

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHS1993

   Plasmid

   #239836

   Purpose

   OMEGAOff-KRK mammalian expression

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   DNMT3A-DNMT3L-OrufIscB-KRK-KRAB
   

   Tags

   HA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Insertion of REC domain from NbaCas9-1 + E137K/E4…

   Promoter

   CMV

   Available Since

   July 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-AURKB

   Plasmid

   #23682

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   AURKB (AURKB Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. R777-E318 Hs.SOS1-nostop

   Plasmid

   #70602

   Purpose

   Gateway ORF clone of human SOS1 [NM_005633.3] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   SOS1 (SOS1 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. vGlut1 peptide- GST

   Plasmid

   #218677

   Purpose

   Expresses a GST fusion to vGlut peptide for immunoisolation of glutamatergic vesicles

   Depositor

   Axel Brunger

   Article

   Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

   Insert

   vGlut (Slc17a6 Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. KW57_HART_RAC1

   Plasmid

   #207399

   Purpose

   Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR

   Depositor

   Stephen Floor

   Article

   Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

   Insert

   eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mRFP-UtrCH

   Plasmid

   #26739

   Depositor

   William Bement

   Article

   Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

   Insert

   Utrophin
   

   Use

   Reporter for f-actin

   Tags

   monomeric RFP

   Expression

   Mammalian

   Mutation

   Calponin homology domain of utrophin (aa# 1-261)

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHDR-USR-eGFP

    Plasmid

    #208830

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV, U6

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 HA Arf6 DN T27N

    Plasmid

    #10831

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 T27N (ARF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T27N. Defective in GTP binding. Dominant negati…

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-CD28-mCherry

    Plasmid

    #223593

    Purpose

    Lentiviral expression of human CD28 with mCherry tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mCherry

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL1425 (pSPIN, pCDF backbone)

    Plasmid

    #160735

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-MARK3-CR

    Plasmid

    #229422

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MARK3-CR (MARK3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    silent mutation CRISPR resistant

    Promoter

    EFS

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-LUC2CP/intron/ARE

    Plasmid

    #62858

    Purpose

    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

    Depositor

    Gideon Dreyfuss

    Article

    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

    Insert

    Luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    insertion of chimeric intron at nueotide poclsiti…

    Promoter

    CMV

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_867

    Plasmid

    #231109

    Purpose

    A>G base editor

    Depositor

    John Doench, David Root

    Insert

    ABE8e-nCas9-SpG-HA
    

    Use

    CRISPR and Lentiviral

    Mutation

    D10A

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-cT3G-cERP2-JUNB

    Plasmid

    #192907

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of JUNB

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    JUNB (JUNB Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMaCTag-Z02

    Plasmid

    #120045

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    TagBFP
    

    Use

    Pcr template

    Tags

    TagBFP

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_ 2xFLAG-2xSTREP_NRF2

    Plasmid

    #159129

    Purpose

    expresses NRF2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    NRF2 (NFE2L2 Human)
    

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. GADD45 WT-luc

    Plasmid

    #8356

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    GADD45 promoter WT (GADD45A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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