163,331 results

1. pX330-PITCh-H2BC11

   Plasmid

   #207755

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a_mCherry_P2A_Hygro

   Plasmid

   #135003

   Purpose

   Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCXIP EGFP-ER [TM(SAC1)]

   Plasmid

   #186475

   Purpose

   Expression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   SAC1 (SACM1L Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C-terminal transmembrane region of the protein: 5…

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. APEX2-csGBP

   Plasmid

   #108874

   Purpose

   Expression of APEX2 fused to a conditionally stable nanobody against GFP

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   APEX2-csGBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. VfAU1-LOV_P2A_casp9

   Plasmid

   #131224

   Purpose

   Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   VfAU1-LOV-HA_P2A_myc-casp9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-flag-YTHDF2

   Plasmid

   #52300

   Purpose

   full length YTHDF2 with N-terminal flag tag for mammalian cell expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGW1-Myc-DJ1-WT

   Plasmid

   #29347

   Depositor

   Mark Cookson

   Article

   Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

   Insert

   DJ1 (PARK7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. plentiCMV-sp-HPV-TM(ICAM)

   Plasmid

   #196510

   Purpose

   Express HPV antigen epitope with ICAM transmembrane domain

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   a HPV epitope with CD8a stalk linker and ICAM TM domain
   

   Use

   Lentiviral

   Tags

   3xflag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-PBZ-mRuby2

   Plasmid

   #110650

   Purpose

   expresses a PAR-binding (PBZ) domain fused to mRuby2

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

   Insert

   PBZ domain from APLF, amino acids 371-451 (APLF Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC034 - LwCas13a-msfGFP-2A-Blast

    Plasmid

    #91924

    Purpose

    Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAW63.YY1.FKBP.knock-in.BFP

    Plasmid

    #104371

    Purpose

    For knocking in FKBP F36V P2A BFP

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    FKBP12(F36V) -P2A-BFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF2925

    Plasmid

    #143676

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZFP57 (ZFP57 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PEmaxRNaseHtrunc

    Plasmid

    #207858

    Purpose

    Mammalian expression of PEmaxDRNaseH prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PEmaxDRNaseH
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-GRAB_r5-HT1.0

    Plasmid

    #208719

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HT1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AiP15578 - pAAV-AiE0780m-minBG-iCre(R297T)-BGHpA (Alias: CN5578)

    Plasmid

    #224200

    Purpose

    AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKM7

    Plasmid

    #154164

    Purpose

    mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 HA Arf6 DN T27N

    Plasmid

    #10831

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 T27N (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T27N. Defective in GTP binding. Dominant negati…

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-tag

    Plasmid

    #55182

    Purpose

    HA-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    HA tag
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. moxCerulean3

    Plasmid

    #68066

    Purpose

    C-terminal fusion to moxCerulean3

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    moxCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPYL-ABI

    Plasmid

    #233650

    Purpose

    Cellular colocalization assay (PYL/ABI) for the CIP OP (opabactin) or the CIP ABA (abscisic acid)

    Depositor

    Richard Wombacher

    Article

    Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

    Insert

    TOMM20-mCherry-PYL-P2A-eGFP-ABI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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