164,909 results

1. p006-GFP-strong

   Plasmid

   #108313

   Purpose

   GFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   Green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pFAB4026

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PermaPhos Kit

   Plasmid Kit

   #1000000226

   Purpose

   This kit is used for expressing recombinant proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) in an engineered self-sufficient E. coli genetic code expansion (GCE) expression system

   Depositor

   Ryan Mehl

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

3. wt HA-dynamin 1 pcDNA3.1

   Plasmid

   #34682

   Depositor

   Sandra Schmid

   Insert

   dynamin 1ba (DNM1L Human)
   

   Use

   Tags

   HA (variant)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. hFLT1

   Plasmid

   #83435

   Purpose

   human VEGFR1 with HA and FLAG tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

   Insert

   human VEGFR1 (FLT1 Human)
   

   Use

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   HA-tag was inserted 30 AA downstream of the FLT1 …

   Promoter

   CMV

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF0373

   Plasmid

   #141594

   Purpose

   Lentiviral vector for overexpressing the NFYA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFYA (NFYA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-NSP1SA11

   Plasmid

   #89168

   Purpose

   Express Rotavirus SA11 NSP1 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 NSP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Mouse genome-wide lentiviral CRISPR gRNA library version 2

   Pooled Library

   #67988

   Purpose

   Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Species

   Homo sapiens, Mus musculus, and Synthetic

   Use

   CRISPR and Lentiviral

   Available since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA-LoxP-mKate-LoxP-EGFP

   Plasmid

   #167940

   Purpose

   CMV promoter-LoxP-mKate-LoxP-EGFP

   Depositor

   Masaharu Somiya

   Article

   Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

   Insert

   LoxP-mKate-LoxP-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Halo-MTS

   Plasmid

   #124315

   Purpose

   Expresses mitochondria specific HaloTag protein

   Depositor

   Jin Wang

   Article

   Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

   Insert

   Halo tag
   

   Use

   Tags

   Mito specific seq

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRXIP VPS16-GFP

    Plasmid

    #67023

    Purpose

    Human VPS16 ORF was inserted into pMRXIP GFP-N vector

    Depositor

    Noboru Mizushima

    Article

    Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

    Insert

    Homo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

11. MSCV-N BNLF2a

    Plasmid

    #37941

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    BNLF2a (BNLF2a H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEZYflag

    Plasmid

    #18700

    Purpose

    pFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-MTS-jAspSnFR3-mRuby3

    Plasmid

    #203492

    Purpose

    Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    MTS-jAspSnFR3-mRuby3
    

    Use

    Lentiviral

    Tags

    His-tag, Mitochondrial localization signal, and m…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJL-TRBO-G

    Plasmid

    #80083

    Purpose

    An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.

    Depositor

    John Lindbo

    Article

    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138131

    Purpose

    Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mCherry-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX302

    Plasmid

    #25896

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. Human DNA Binding Domain-Focused CRISPR Knockout Library

    Pooled Library

    #123334

    Purpose

    Expresses gRNA targeting the DNA binding domain of human transcription factors.

    Depositor

    Christopher Vakoc

    Article

    Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

18. pFW6000

    Plasmid

    #224215

    Purpose

    Bacteroides genomic editing tool; aTc inducible-Fncpf1

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHIS3p:CloverGFP-Tub1+3'UTR::URA3

    Plasmid

    #50636

    Depositor

    Wei-Lih Lee

    Article

    Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

    Insert

    CloverGFP-Tub1
    

    Use

    Yeast expression

    Tags

    CloverGFP

    Expression

    Mutation

    Promoter

    HIS3p

    Available since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF3081

    Plasmid

    #144557

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ELK3 (ELK3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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