178,550 results
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Plasmid#105321PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoterDepositorHas serviceAAV1InsertjGCaMP7c variant 1513
UseAAVTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterSynapsinAvailable sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
ExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB2-E2-Crimson
Plasmid#104010PurposePlasmid encoding E2-CrimsonDepositorInsertE2-Crimson
UseLow copyExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB1-mVenus NB
Plasmid#103986PurposePlasmid encoding mVenus NBDepositorInsertmVenus NB
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0045-EF1a-PguCas13b-NES-HIV
Plasmid#103861PurposeExpresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasmDepositorInsertPguCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LAMP-mCherry-CRY2
Plasmid#102249PurposeExpresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)DepositorAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET15bHis6Tnp
Plasmid#79807PurposeTransform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutationsDepositorInsertHis6Tnp
TagsHisExpressionBacterialAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
px459 VQR
Plasmid#101715PurposesgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)DepositorInsertSpCas9 VQR
Tags3xFLAG-NLS and NLSExpressionMammalianMutationVQR (D1135V, R1335Q and T1337R)Available sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGF1-4eCOL2A1
Plasmid#97210PurposeLentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoterDepositorInsert4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
UseLentiviral and LuciferaseExpressionMammalianPromoterCOL2A1 regulatory elementsAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
IL2Rα-EGFP
Plasmid#86055PurposeA mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.DepositorInsertInterleukin-2 receptor subunit alpha (IL2RA Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-FLAG-ATP6V0D1
Plasmid#87931PurposeFLAG-tagged overexpressionDepositorAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ220 (AsCpf1)
Plasmid#86208PurposeAsCpf1 Gateway entry plasmidDepositorInsertAsCpf1
UseGateway compatible cpf1 vectorsTags5' and 3' NLSExpressionPlantAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ233 (dLbCpf1-SRDX)
Plasmid#86211PurposeDeactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPRTags5' and 3' NLS and SRDX repressorExpressionPlantAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-1BPNLS-Cas9-1BPNLS
Plasmid#87108PurposeBPNLS-Cas9-BPNLS expression plasmidDepositorInsert1BPNLS-Cas9-1BPNLS
UseCRISPRTags3xFLAGExpressionMammalianPromoterCAGAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV9:cTNT::3Flag-hYAP S127A
Plasmid#86558PurposeTo produce cardiac specific adeno-associated virus expressing activated human YAP.DepositorInsertYAP (YAP1 Human)
UseAAVTags3FlagMutationSerine 127 was mutated into Alanine, to activate …PromotercTNTAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV Marina-T2A-nls-mCherry
Plasmid#74216Purposegenetically-encoded fluorescent voltage indicatorDepositorInsertfluorescent protein voltage sensor
TagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAW016-2
Plasmid#85613PurposeCas9-tracrRNA integration vector for Bacillus subtilisDepositorInsertcas9-tracrRNA
ExpressionBacterialAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti Lifeact-mRuby2 BlastR
Plasmid#84384PurposeLentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmRuby2PromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-dGBP1-TagBFP
Plasmid#80086PurposeGFP-dependent dGBP1-TagBFP, for expression in mammalian cellsDepositorInsertdGBP1-TagBFP
ExpressionMammalianPromoterCAGAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by