|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20156||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4733
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameCFP-FKBP-Inp54p (D281A)
SpeciesS. cerevisiae (budding yeast)
MutationStop codon at position 331. D281A mutation.
Entrez GeneINP54 (a.k.a. YOL065C)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CF-Inp(D281A) was a gift from Tobias Meyer (Addgene plasmid # 20156 ; http://n2t.net/addgene:20156 ; RRID:Addgene_20156)
For your References section:Rapid chemically induced changes of PtdIns(4,5)P2 gate KCNQ ion channels. Suh BC, Inoue T, Meyer T, Hille B. Science. 2006 Dec 1. 314(5804):1454-7. 10.1126/science.1131163 PubMed 16990515