|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20117||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerP. Ortiz de Montellano, UCSF
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameAmorphadiene oxidase and p450 reductase
Alt nameAMO, CPR
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCWori-A13AMO-aaCPRct was a gift from Jay Keasling (Addgene plasmid # 20117 ; http://n2t.net/addgene:20117 ; RRID:Addgene_20117)
For your References section:Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Chang MC, Eachus RA, Trieu W, Ro DK, Keasling JD. Nat Chem Biol. 2007 May . 3(5):274-7. 10.1038/nchembio875 PubMed 17438551