PurposeBglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47050||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2300
- Total vector size (bp) 6656
Vector typeBacterial Expression, Synthetic Biology ; BglBrick
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
Insert Size (bp)4358
- Promoter Pbad
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI, BglII (not destroyed)
- 3′ cloning site BamHI, XhoI (not destroyed)
- 5′ sequencing primer F8
- 3′ sequencing primer R1 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Use a low concentration of Kanamycin (12.5 micrograms/mililiter) for perillyl alcohol production
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJBEI-6411 was a gift from Taek Soon Lee (Addgene plasmid # 47050 ; http://n2t.net/addgene:47050 ; RRID:Addgene_47050)
For your References section:Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold CJ, Lee TS. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. 10.1016/j.ymben.2013.05.004 PubMed 23727191