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pJBEI-6411
(Plasmid #47050)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47050 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBbB8k
  • Backbone size w/o insert (bp) 2300
  • Total vector size (bp) 6656
  • Vector type
    Bacterial Expression, Synthetic Biology ; BglBrick

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
  • Species
    Sphingomonas
  • Insert Size (bp)
    4358
  • Promoter Pbad

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI, BglII (not destroyed)
  • 3′ cloning site BamHI, XhoI (not destroyed)
  • 5′ sequencing primer F8
  • 3′ sequencing primer R1
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Use a low concentration of Kanamycin (12.5 micrograms/mililiter) for perillyl alcohol production

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJBEI-6411 was a gift from Taek Soon Lee (Addgene plasmid # 47050 ; http://n2t.net/addgene:47050 ; RRID:Addgene_47050)
  • For your References section:

    Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold CJ, Lee TS. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. 10.1016/j.ymben.2013.05.004 PubMed 23727191