167,564 results

1. pCEFLmGFP-Gli2

   Plasmid

   #37672

   Depositor

   Philip Beachy

   Article

   Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

   Insert

   Gli2 (Gli2 Mouse)
   

   Tags

   mGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAWPSG-Po-nCas9-BE

   Plasmid

   #195740

   Purpose

   Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Po (phoenol-inducible promoter)

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FKBP-CD28TMD-NTEVp(H75S)

   Plasmid

   #137828

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(H75S)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Histidine 75 to Serine in NTEVp fragment

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHM066

   Plasmid

   #225524

   Purpose

   Encodes Wnt-responsive destablized iRFP

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Insert

   iRFP
   

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mRFP-Rab7

   Plasmid

   #14436

   Purpose

   Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

   Depositor

   Ari Helenius

   Article

   Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

   Insert

   Rab 7 (RAB7A Canis familialis)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   3 silent mutations near the C-terminus

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-flag-GFP-iCAM-1

   Plasmid

   #205186

   Purpose

   This vector encodes of a synCAM (iCAM1) and GFP

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM iCAM1 and GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EgI-HygroR

   Plasmid

   #207177

   Purpose

   Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-HygroR

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEFIRES-P-EGFP-ADRP

   Plasmid

   #87157

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   mCherry-R TTGGTCACCTTCAGCTTGG

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1+DPP6

   Plasmid

   #117272

   Purpose

   Encodes human DPP6 variant 1

   Depositor

   Mark Cannell

   Article

   Lainez et al Biochem Pharmacol. 2018 Apr;150:120-130.

   Insert

   DPP6 (DPP6 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-cT3G-cERP2-FOS

    Plasmid

    #192924

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of FOS

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOS (FOS Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-NLS-GFP

    Plasmid

    #104061

    Purpose

    AAV expression of nuclear localized GFP with SV40 NLS

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV PHP.eB

    Insert

    GFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CL20_mEGFP_ERBB2_PPP1R1B

    Plasmid

    #205813

    Purpose

    Express mEGFP-tagged fusion protein, ERBB2_PPP1R1B from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    ERBB2_PPP1R1B (ERBB2, PPP1R1B Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-CAG-FLPX-rc [Jaws-KGC-tdTomato-ER2]

    Plasmid

    #195488

    Purpose

    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in FLPX/reversed (Frt-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CAG

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX458-Ef1a-Cas9-H2B-mCherry

    Plasmid

    #159655

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCCL/IFNB1-d2eGFP-3’UTR

    Plasmid

    #180232

    Purpose

    Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

    Insert

    d2eGFP
    

    Use

    Lentiviral

    Promoter

    1 kb upstream of IFNB1 CDS

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBad-EBFP2

    Plasmid

    #14891

    Purpose

    Bacterial expression of EBFP2 blue fluorescent protein

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 May 22;46(20):5904-10

    Has Service

    Cloning Grade DNA

    Insert

    EBFP2
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    The fluorescent protein was inserted between XhoI…

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid

    #71657

    Purpose

    mammalian expression of eiF4A1

    Depositor

    Gideon Dreyfuss

    Insert

    eiF4A1 (EIF4A1 Human)
    

    Tags

    HIS and myc

    Expression

    Mammalian

    Mutation

    aa403 from Ala to Arg

    Promoter

    CMV

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. GCH1_pLX307

    Plasmid

    #98336

    Purpose

    Lentiviral expression of GCH1

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    GCH1 (GCH1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG mito-RCaMP1h

    Plasmid

    #105013

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-Kif2A

    Plasmid

    #52401

    Purpose

    Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

    Depositor

    Gohta Goshima, Ryota Uehara

    Article

    Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

    Insert

    Kif2A (KIF2A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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