163,398 results

1. pYTK095

   Plasmid

   #65202

   Purpose

   Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMALB-ATF4.14

   Plasmid

   #155034

   Purpose

   Fluorescent reporter for ATF4 translation, Positive Control

   Depositor

   John Dick, Peter van Galen

   Article

   van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

   Inserts

   ATF4 5' UTR (Atf4 Mouse)
   ATF4-eGFP fusion ORF
   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   uORF1 and uORF2 start codons mutated

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV R-CEPIA3mt

   Plasmid

   #140464

   Purpose

   Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

   Depositor

   Masamitsu Iino

   Article

   Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

   Insert

   R-CEPIA3mt
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCETT

   Plasmid

   #19782

   Depositor

   Jumi Shin

   Article

   Chen et al Biotechniques. 2008 Sep . 45(3):295-304.

   Type

   Empty backbone

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

   Plasmid

   #44028

   Depositor

   Celeste Simon

   Article

   Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

   Insert

   mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P402A, P577A, N813A

   Promoter

   CMV

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSPTg.T2FpAXK (#52)

   Plasmid

   #35962

   Depositor

   Thomas Sato

   Article

   Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

   Inserts

   Tie2 promoter
   SV40 pA
   Tie2 enhancer
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUP-(2xCouRS-tRNAopt)

   Plasmid

   #197930

   Purpose

   Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

   Inserts

   CouRS
   tRNA opt
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   glnS' and lambda(ts) and proK

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TA-ERP2

   Plasmid

   #80477

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. ILF2_DZF_pGEX

   Plasmid

   #135162

   Purpose

   Recombinant expression of ILF2 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   ILF2 (ILF2 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. R777-E231 Hs.RASA3

    Plasmid

    #70515

    Purpose

    Gateway ORF clone of human RASA3 [NM_007368.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASA3 (RASA3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pnCS_SEPT7_SEPT9_i3-TEV-Strep

    Plasmid

    #174501

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i3

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PEmaxNG-_2star_IVT

    Plasmid

    #227678

    Purpose

    plasmid for SpCas9-NG-PEmax** mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    NG-PEmax_2_star
    

    Use

    CRISPR

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAW-Nb127D01-GFP

    Plasmid

    #171571

    Purpose

    Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cells

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-GFP (CXCR2 Human)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTR-346 pUC19-tNGFR-P2A-IL2RA N

    Plasmid

    #186065

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL2RA (IL2RA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-(SUMO)3

    Plasmid

    #127093

    Purpose

    Bacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    EGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIG-FGFRdn

    Plasmid

    #80431

    Purpose

    Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

    Depositor

    Aixa Morales

    Article

    Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

    Insert

    Fibroblast growth factor receptor 1 (FGFR1 Chicken)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains aa 1–425

    Promoter

    beta-actin promoter

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

17. Zinc Finger Consortium Expression Vector Kit v1.0

    Plasmid Kit

    #1000000010

    Purpose

    Contains plasmids and strains for the bacterial two-hybrid (B2H) system used to screen zinc finger arrays for activity. Also called the Modular Assembly Accessory Reagents Kit.

    Depositor

    Keith Joung

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pSico

    Plasmid

    #11578

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-PIG-miR-146a

    Plasmid

    #64234

    Purpose

    Expresses miR-146a in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

    Insert

    miR146a (MIR146A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-myc-ACE2

    Plasmid

    #141185

    Purpose

    Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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