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pET28a-MH6-RspCas13d_RspCasWYL1
(Plasmid #108304)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108304 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5390
  • Total vector size (bp) 9420
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    RspCas13d
  • Species
    Synthetic
  • Insert Size (bp)
    2763
  • Promoter lac
  • Tag / Fusion Protein
    • mH6 tag (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ATGGCCAAAAAGAACAAAATGAAACC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    RspWYL1
  • Species
    Synthetic
  • Insert Size (bp)
    1182
  • Tag / Fusion Protein
    • mH6 tag (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer ATGCTGATTCCGCCTAGCAC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

For commercial use, please contact Arbor Biotechnologies at [email protected].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-MH6-RspCas13d_RspCasWYL1 was a gift from Arbor Biotechnologies (Addgene plasmid # 108304 ; http://n2t.net/addgene:108304 ; RRID:Addgene_108304)
  • For your References section:

    Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein. Yan WX, Chong S, Zhang H, Makarova KS, Koonin EV, Cheng DR, Scott DA. Mol Cell. 2018 Mar 9. pii: S1097-2765(18)30173-4. doi: 10.1016/j.molcel.2018.02.028. 10.1016/j.molcel.2018.02.028 PubMed 29551514