163,398 results

1. pMG343

   Plasmid

   #176183

   Purpose

   preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   TPK2+ccdB dropout cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Syn1-tTA

   Plasmid

   #104109

   Purpose

   AAV-mediated expression of tTA under the Syn1 promoter.

   Depositor

   Takeshi Imai

   Article

   Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

   Insert

   tTA2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn1

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH_blast_MCS_Nard_GFP_LAMIN

   Plasmid

   #167340

   Purpose

   Expresses GFP fused to lamin A (LA) in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103_TP53BP2-1/1

   Plasmid

   #91503

   Purpose

   Protein expression and purification of human SH3 domain construct TP53BP2-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   TP53BP2-1/1 (TP53BP2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO HIS HSPA8

   Plasmid

   #19541

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   heat shock 70kDa protein 8 (HSPA8 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF5B-FRT-cilia-APEX

   Plasmid

   #73186

   Purpose

   Expresses cilia-APEX in mammalian cells

   Depositor

   Maxence Nachury

   Article

   Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

   Insert

   cilia-APEX (Nphp3 Glycine max, Mouse)
   

   Use

   Tags

   internal GFP tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p667-UBC-VRK3-V5-miniTurbo_IDG-K

   Plasmid

   #189902

   Purpose

   Gateway destination clone of VRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   VRK3 (VRK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Keasling-2337

   Plasmid

   #100168

   Purpose

   Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

   Depositor

   Jay Keasling

   Article

   Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

   Insert

   lacIq-Ptrc-ADS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Hygro EYFP H148Q/I152L

   Plasmid

   #25874

   Depositor

   Peter Haggie

   Article

   Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

   Insert

   EYFP H148Q/I152L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EYFP from pEYFP (Clontech) with H148Q and I152L m…

   Promoter

   Available since

   Aug. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

    Plasmid

    #164510

    Purpose

    Green glucose sensor with mRuby2 fusion, cytosolic

    Depositor

    Loren Looger

    Article

    Keller et al bioRxiv (2019) 571422; doi: https://doi.

    Has Service

    AAV9

    Insert

    (Cyto)iGlucoSnFR-mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Synthetic Construct

    Promoter

    Synapsin

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIG3 (pCMV-IRES-GFP version 3)

    Plasmid

    #78264

    Purpose

    Lentiviral empty cloning vector containing IRES and GFP

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPAGFP-VSVG

    Plasmid

    #11915

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    (L64F/T65S/V163A/T203H). The PAGFP also contains …

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tubulin-mGreenLantern

    Plasmid

    #164460

    Purpose

    Localization of mGreenLantern fluorescent protein to tubulin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Tubulin

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKRaION2-N1

    Plasmid

    #177802

    Purpose

    Mammalian expression of green fluorescent potassium indicator KRaION2

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLAMP1-emiRFP670

    Plasmid

    #136570

    Purpose

    LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    LAMP1-emiRFP670
    

    Use

    Tags

    emiRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX551-CMV-SpCas9

    Plasmid

    #107024

    Purpose

    AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab

    Depositor

    Alex Hewitt

    Insert

    SpCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUK21

    Plasmid

    #49788

    Purpose

    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2-CIB1-mCherry-Rab8A

    Plasmid

    #194333

    Purpose

    For use in light-induced Rab8a protein inactivation through the interaction with CRY2

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Inserts

    CIB1 (CIB1 Mustard Weed)
    Rab8a (RAB8A Canis Lupus)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GW1-Peredox-mCitrine

    Plasmid

    #32386

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCitrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid

    #177672

    Purpose

    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells

    Depositor

    Karl Deisseroth

    Has Service

    AAV8

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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