182,244 results
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Plasmid#103986PurposePlasmid encoding mVenus NBDepositorInsertmVenus NB
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET15bHis6Tnp
Plasmid#79807PurposeTransform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutationsDepositorInsertHis6Tnp
TagsHisExpressionBacterialAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
px459 VQR
Plasmid#101715PurposesgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 VQR
Tags3xFLAG-NLS and NLSExpressionMammalianMutationVQR (D1135V, R1335Q and T1337R)Available sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4-AARE-luc2P-Hygro
Plasmid#101787PurposeLuferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xAARE
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZac2.1 GfaABC1D hM3D mCherry SV40
Plasmid#92284PurposeAAV DREADD expressionDepositorAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBluescript-CTG-47
Plasmid#99150Purpose47xCAG repeats in pBluescript vectorDepositorInsert47xCTG
ExpressionBacterialPromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSFFV_sfCherry2(11)_TagBFP
Plasmid#82606PurposeExpresses sfCherry2(11) and TagBFP fusion in mammalian cellsDepositorInsertsfCherry2(11)_TagBFP
UseLentiviralExpressionMammalianMutationchanged Gly12 to Ala in sfCherry11+PromoterSFFVAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-Tau
Plasmid#92201PurposeRetroviral overexpression vector (doxycycline-inducible) for TauDepositorAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK302
Plasmid#87768PurposeAs pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be usedDepositorInsertsPrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli
UseSynthetic BiologyTags6xHis TagExpressionBacterialPromoterPrhaBAD rhamnose-inducible promoter from E. coli …Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-ATP6V0D1
Plasmid#87931PurposeFLAG-tagged overexpressionDepositorAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ220 (AsCpf1)
Plasmid#86208PurposeAsCpf1 Gateway entry plasmidDepositorInsertAsCpf1
UseGateway compatible cpf1 vectorsTags5' and 3' NLSExpressionPlantAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ233 (dLbCpf1-SRDX)
Plasmid#86211PurposeDeactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPRTags5' and 3' NLS and SRDX repressorExpressionPlantAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-1BPNLS-Cas9-1BPNLS
Plasmid#87108PurposeBPNLS-Cas9-BPNLS expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert1BPNLS-Cas9-1BPNLS
UseCRISPRTags3xFLAGExpressionMammalianPromoterCAGAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFG2
Plasmid#87250Purposegene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-NatDepositorInsertNat
TagsparS segmentsExpressionYeastPromoterTEFAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV9:cTNT::3Flag-hYAP S127A
Plasmid#86558PurposeTo produce cardiac specific adeno-associated virus expressing activated human YAP.DepositorInsertYAP (YAP1 Human)
UseAAVTags3FlagMutationSerine 127 was mutated into Alanine, to activate …PromotercTNTAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pF CAG luc puro
Plasmid#67501PurposeConstitutive expression of firefly luciferase in mammalian cellsDepositorInsertFirefly luciferase
UseLentiviral and LuciferaseExpressionMammalianPromoterCAGAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCX-DI-2A
Plasmid#74671PurposeMammalian expression of DI-2A (hCD73/F2A/hCD39)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsF2AExpressionMammalianPromoterpCAGGSAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 mCherry-Atl3
Plasmid#86680PurposeLentivirus to stably express fluorescent protein in mammalian cellsDepositorAvailable sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV Marina-T2A-nls-mCherry
Plasmid#74216Purposegenetically-encoded fluorescent voltage indicatorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertfluorescent protein voltage sensor
TagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAW016-2
Plasmid#85613PurposeCas9-tracrRNA integration vector for Bacillus subtilisDepositorInsertcas9-tracrRNA
ExpressionBacterialAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by