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Plasmid#228065PurposeExpresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.DepositorInsertNAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
UseDexamethasone inducible vectorTagsExpressionPlantMutationPromoterCaMV35S for LhGR and pOp6-minimal CaMV35S for NST1Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKAN43Ag8-GFP
Plasmid#178182PurposePlant binary vector expressing GFP by a enhanced 35S promoterDepositorInsertEGFP (eGFP )
UseTagsExpressionPlantMutationThe CAT is inserted after the start codon.PromoterThe minimal 35S promoter from Cauliflower Mosaic …Available sinceSept. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0130
Plasmid#143013PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHMGB3 (HMGB3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
4A3.PBAD-repL(WT).J23115-gfp
Plasmid#197872PurposeArabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expressionDepositorInsertspart kilA/repL
gfp
UseTagsExpressionBacterialMutationcontains only the 24 bp of 3'-end of kilA ge…PromoterBba_J23115 and PBADAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
4A3.PBAD-repL(AT2).J23115-gfp
Plasmid#197873PurposeArabinose induced expression of repL(AT2) gene, which triggers DNA replication in trans, weak level of constitutive GFP expressionDepositorInsertpart kilA/repL(AT2)
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPE1_Z
Plasmid#148115PurposeMammalian Expression of HshnRNPE1DepositorInsertHshnRNPE1 (PCBP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMX-SHP1C/S-ires-mCD8
Plasmid#183719PurposeExpresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) (substrate-trapping mutant) and mouse CD8.DepositorInsertSHP-1
UseRetroviralTagsmycExpressionMutationC453SPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO SnRK2.6 (11-362)
Plasmid#177847PurposeBacterial Expression of SnRK2.6DepositorInsertSNF1-related protein kinase 2.6 (OST1 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
TANGO1S-mScarlet-i
Plasmid#165461PurposeEncodes TANGO1S tagged with mScarlet-iDepositorInsertTANGO1S
UseTagsmScarlet-iExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS185 CMV-Cre
Plasmid#11916PurposeMammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoterDepositorInsertcre (cre bacteriophage P1)
UseCre/LoxTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
ETS1_pet28a
Plasmid#131663PurposeBacterial expression of ETS gene ETS1DepositorInsertETS1 (ETS1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)
Plasmid#101178PurposeHuman expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationN692A/M694A/Q695A/H698APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOS011: superecliptic pHluorin (mitochondrial)
Plasmid#163050Purposesuperecliptic pHluorin (mitochondrial)DepositorInsertsuperecliptic pHluorin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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FNLCR CRISPRa Cell Surface and FNLCR CRISPRa Soluble Libraries
Pooled Library#1000000228DepositorAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EBFP2-nuc
Plasmid#36085DepositorTypeEmpty backboneUseLentiviralTagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…PromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
dPspCas13b-mutFTO
Plasmid#223697PurposeExpressing fusion protein of dPspCas13b and inactive mutant of human FTODepositorInsertdPspCas13b-GGS-mutFTO (FTO Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
B-95.ΔA
Bacterial Strain#197933PurposeBL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acidsDepositorBacterial ResistanceNoneAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir
Plasmid#90009PurposeSplit-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBPDepositorInsertsFKBP-linker-Myc-NBirA*
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only