163,331 results

1. pLenti-Puro-sgNUAK2

   Plasmid

   #138693

   Purpose

   Expresses a human NUAK2-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgNUAK2 human (NUAK2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCap-SG5-Apr

   Plasmid

   #227579

   Purpose

   Capture plasmid with apramycin resistance and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. IGF1R_pLX307

   Plasmid

   #98344

   Purpose

   Lentiviral expression of IGF1R

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   IGF1R (IGF1R Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. PARP1 FL

   Plasmid

   #169815

   Purpose

   Expresses codon optimised full length His tagged PARP1 in bacterial cells

   Depositor

   David Neuhaus

   Article

   Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

   Insert

   Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Human, Synthetic)
   

   Use

   Tags

   MKHHHHHHMKQ

   Expression

   Bacterial

   Mutation

   Valine 762 mutated to an alanine

   Promoter

   T7 promoter

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LV-CFP

   Plasmid

   #25998

   Depositor

   Elaine Fuchs

   Article

   Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

   Insert

   H2B-CFP (H2BC21 Human, A. victoria)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pR26

   Plasmid

   #127372

   Purpose

   Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   tight TRE promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BE4-Gam

   Plasmid

   #100806

   Purpose

   Expresses BE4-Gam in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

   Insert

   BE4-Gam
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Pramr-GFP

   Plasmid

   #194148

   Purpose

   Contains a RamR-regulated promoter expressing GFP

   Depositor

   Andrew Ellington

   Article

   d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pramr

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-sfGFP

   Plasmid

   #125747

   Purpose

   sfGFP without iCreERT2 fusion

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. talin1-head

    Plasmid

    #182996

    Purpose

    expression of talin1 head wt in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    talin1 head (Tln1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag-GFP-TurboID-NES_pLX304

    Plasmid

    #202019

    Purpose

    expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    Flag-GFP-TurboID-NES
    

    Use

    Lentiviral

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223-CAMK2B

    Plasmid

    #23820

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CAMK2B (CAMK2B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. BIN1-pmCherryN1

    Plasmid

    #27693

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    BIN1 (Bin1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

14. qi5

    Plasmid

    #24501

    Depositor

    Bruce Conklin

    Article

    Conklin et al Nature. 1993 May 20. 363(6426):274-6.

    Insert

    Gq alpha (Gnaq Mouse)
    

    Use

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    Gq alpha with the C-terminal amino acids changed …

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pClneoMyc-LATS2

    Plasmid

    #66852

    Purpose

    Express Myc-tagged LATS2 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    LATS2 (LATS2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-hULK1

    Plasmid

    #31963

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK1 (ULK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSerOTS-C1* (V70)

    Plasmid

    #188537

    Purpose

    Phosphoserine incorporation into recombinant proteins

    Depositor

    Jesse Rinehart

    Article

    Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

    Inserts

    pSerRS
    Ef-pSer
    tRNApSer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations resulting from laboratory evolution

    Promoter

    glnS*

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pR-spondin 1

    Plasmid

    #128844

    Purpose

    bacterial expression of pR-spondin 1

    Depositor

    Marc de la Roche

    Article

    Urbischek et al Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.

    Insert

    R-spondin 1 (RSPO1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF3173

    Plasmid

    #144649

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHaMDRwt

    Plasmid

    #10957

    Depositor

    Michael Gottesman

    Article

    Pastan et al Proc Natl Acad Sci U S A. 1988 Jun . 85(

    Insert

    MDR1 (ABCB1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge