167,564 results

1. DD-Cas9 with filler sequence and Venus (EDCPV)

   Plasmid

   #90085

   Purpose

   This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA

   Depositor

   Raffaella Sordella

   Article

   Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized Domain and Flag

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH3U3-zif268 (omega)

   Plasmid

   #18046

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

   Insert

   zif268 binding site
   

   Expression

   Bacterial

   Mutation

   The preferred binding site for zif268 is inserted…

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSJ95

   Plasmid

   #104343

   Purpose

   Express yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Chen et al Science. 2017 Jan 27;355(6323). pii: 355

   Insert

   FBP1
   

   Tags

   3xFLAG

   Expression

   Yeast

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. fwYellow chromoprotein

   Plasmid

   #117841

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, fwYellow
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 9ao-SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)

   Plasmid

   #209293

   Purpose

   mitoBE expression vector, target mtND4 gene.

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

   Insert

   SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDY0209 ACTB Nicking guide +48

   Plasmid

   #179109

   Purpose

   ACTB N-term Nicking guide 1 +48 guide

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   ACTB N-term Nicking guide 1 +48 guide
   

   Use

   CRISPR

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-mTagBFP-CAAX

   Plasmid

   #75149

   Purpose

   Membrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   mTagBFP-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. R777-E100 Hs.GRB2-nostop

   Plasmid

   #70384

   Purpose

   Gateway ORF clone of human GRB2 [NM_002086.4] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   GRB2 (GRB2 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-pHuji

   Plasmid

   #61555

   Purpose

   Red fluorescent protein based pH indicator in bacterial expression vector

   Depositor

   Robert Campbell

   Article

   Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

   Insert

   pHuji
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-FLT3-D835Y-V5/HIS

    Plasmid

    #236007

    Purpose

    expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    D835Y substitution

    Promoter

    CMV

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. R777-E126 Hs.MAPK1-nostop

    Plasmid

    #70410

    Purpose

    Gateway ORF clone of human MAPK1 [NM_002745.4] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKL002

    Plasmid

    #98921

    Purpose

    pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Inserts

    PROPS
    GCaMP6f
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Syn-ChrimsonR-GFP

    Plasmid

    #58806

    Purpose

    AAV expression of ChrimsonR-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    Syn

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. PMXS-NDI1

    Plasmid

    #72876

    Purpose

    expresses NDI1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

    Insert

    NDI1 (NDI1 Budding Yeast)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. T7-CMVtrans-FFLuc-polyA

    Plasmid

    #101156

    Purpose

    Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

    Depositor

    Marcel Bruchez

    Article

    Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Promoter

    T7 promoter

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSP1E3

    Plasmid

    #20064

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E3 (APOA1 synthetic gene)
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. p4xSTAT6-Luc2P

    Plasmid

    #35554

    Depositor

    Axel Nohturfft

    Insert

    4xStat6 promoter
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    4xStat6

    Available Since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-IKKbeta (S177E,S181E)-pcw107

    Plasmid

    #64609

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    IKBKB
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    S177E, S181E

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pDONR207-Citrine-NLS

    Plasmid

    #44586

    Purpose

    Gateway entry vector for Citrine fluorescent protein with a nuclear localization signal

    Depositor

    Yutaka Kodama

    Article

    Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

    Insert

    Citrine-NLS
    

    Tags

    NLS (Nuclear Localization Signal)

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Drp1K38A

    Plasmid

    #45161

    Depositor

    Alexander van der Bliek, Richard Youle

    Article

    Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

    Insert

    Drp1 (DNM1L Human)
    

    Tags

    HA (AYPYDVPDY)

    Expression

    Mammalian

    Mutation

    K38A

    Promoter

    CMV

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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