164,909 results

1. pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro

   Plasmid

   #212675

   Purpose

   Express tagged hRb phosphosite mutant

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   15 CDK phospho-sites are mutated to alanines, and…

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-Shank1 [N22/21R] (Recombinant Antibody)

   Antibody

   #209594-rAb

   Purpose

   Anti-Shank1 (Rat) recombinant mouse monoclonal antibody

   Depositor

   James Trimmer

   Article

   Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

   Recommended Applications

   Western Blot

   Reactivity

   Human, Mouse, and Rat

   Source Species

   Mouse

   Isotype

   IgG2a

   Trial Size

   Available to purchase

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

3. pAWO-SUMO-3C_14M_zTDP2

   Plasmid

   #200512

   Purpose

   Expression of zebrafish TDP2 recombinant protein

   Depositor

   Antony Oliver

   Insert

   TDP2 (tdp2b Zebrafish)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   L136N, A139S, C239T, K240R, K309Q, K314L, T315G, …

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD649-HAsp-NCR1-COMP5AP-AviTag-9xHis

   Plasmid

   #157253

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   NCR1 (NCR1 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sender_plasmid2

   Plasmid

   #140693

   Purpose

   Encodes inducible expression of RhlI (C4-HSL quorum sensing synthase)

   Depositor

   Matthew Bennett

   Article

   Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

   Insert

   RhlI (rhlI )
   

   Use

   Tags

   ssrA degradation tag (ASV)

   Expression

   Bacterial

   Mutation

   Promoter

   engineered lac promoter (IPTG inducible)

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. rEstus

   Plasmid

   #209767

   Purpose

   To express a bright and sensitive genetically encoded voltage indicator in mammalian cells.

   Depositor

   Stefan Heinemann

   Article

   Rühl et al bioRxiv 2023.

   Insert

   rEstus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHCMV_DENV2_PrME

   Plasmid

   #207264

   Purpose

   Mammalian expression of Dengue virus 2 PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   DENV2_PrME (POLY Dengue virus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pECE-M2-CDC27 wt

   Plasmid

   #31661

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   cell division cycle 27 homolog (CDC27 Human)
   

   Use

   Tags

   M2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pFUW-VenusFlag-hCD44

   Plasmid

   #211824

   Purpose

   Express CD44 in mammalian cells

   Depositor

   Haian Fu

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   CD44 (CD44 Human)
   

   Use

   Lentiviral

   Tags

   N-Terminal Venus and Flag tags

   Expression

   Mammalian

   Mutation

   isoform corresponds to UniProt ID P16070-1 with a…

   Promoter

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCh-Sec61 beta

    Plasmid

    #49155

    Purpose

    expression of Sec61 beta fused to mCherry, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    mCherry*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HRE-oUnaG

    Plasmid

    #201712

    Purpose

    Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSMALB-ATF4.5

    Plasmid

    #155032

    Purpose

    Fluorescent reporter for ATF4 translation

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGFP

    Plasmid

    #32748

    Depositor

    Jeanne Hardy

    Article

    Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

    Insert

    GFP
    

    Use

    Tags

    DEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRL

    Expression

    Mammalian

    Mutation

    S65T

    Promoter

    CMV

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_BiVIPe4_GCaMP6f

    Plasmid

    #213859

    Purpose

    AAV construct expressing GCaMP6f driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_GCaMP6f

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-nEF-Con/Fon-NpHR3.3-EYFP (AAV8)

    Viral Prep

    #137152-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-NpHR3.3-EYFP (#137152). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-NpHR3.3-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of NpHR3.3-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Cre and Flp-dependent)

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74009

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5x UAS
    Cas9
    T2A
    GFP
    U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189925

    Purpose

    AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SauriABE8e
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SauriCas9 D15A

    Promoter

    EFS

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLBS-mScarlet

    Plasmid

    #129337

    Purpose

    cytosol marker, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    mScarlet (Addgene 85042)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEM319 - array co-injection fragment mosTI unc-119

    Plasmid

    #159823

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI unc-119
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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