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p126

Plasmid

#186176

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 126

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-LAMP1

Plasmid

#222318

Purpose

Synchronize the trafficking of LAMP1 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1 fused to SBP-mCherry (LAMP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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hIRE1a wt

Plasmid

#20744

Depositor

Fumihiko Urano

Article

Lipson et al PLoS ONE. 2008 . 3(2):e1648.

Insert

IRE1 (ERN1 Human)

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_ORI_empty

Plasmid

#99297

Purpose

Luciferase validation vector; empty vector without insert to determine baseline

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Luciferase; Starr-seq luciferase validation vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMSC226

Plasmid

#216375

Purpose

xhNup358-Nb2t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup358-Nb2t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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Emerin pEGFP-N2 (588)

Plasmid

#61985

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

Emerin (EMD Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pN7CAST

Plasmid

#190661

Purpose

Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGCS-N2(3xHA)

Plasmid

#85719

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xHA

Expression

Mutation

Promoter

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-ACSL3-mCherry

Plasmid

#87158

Purpose

Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ACSL3 (ACSL3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF-1-alpha

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST

Plasmid

#125689

Purpose

C to T base editor for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

Insert

streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

ermE*/tipA

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA

Plasmid

#45789

Depositor

Richard Murray

Inserts

deGFP
Tetracycline repressor
araC

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pKL2351

Plasmid

#199721

Purpose

A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

NPTII

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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FUW

Plasmid

#14882

Depositor

David Baltimore

Article

Lois et al Science. 2002 Feb 1. 295(5556):868-72.

Insert

flap-Ub promoter-WRE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only

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PKA-KTR-mStrawberry reporter

Plasmid

#158688

Purpose

This plasmid is a PKA pathway reporter. It has CMV promoter driving the expression of PKA-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-GCaMP6s

Plasmid

#105714

Purpose

Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

Depositor

Rylan Larsen

Has Service

AAV8

Insert

GCaMP6s

Use

AAV; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a, Human elongation factor-1 alpha

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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NUMB2-eGFP-M107K

Plasmid

#37803

Depositor

Rolf Bjerkvig

Article

Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

Insert

NUMB isoform 2 (NUMB Human)

Use

Lentiviral

Tags

eGFP

Expression

Mutation

Promoter

pCMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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p146

Plasmid

#186178

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 146

Use

Synthetic Biology; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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Pan-cancer CasRx lncRNA Albarossa library

Pooled Library

#212966

Purpose

The human CasRx CRISPR lentiviral library encodes 75,725 gRNA arrays (two gRNAs per array) targeting 24,171 lncRNAs.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only
lenti-UCOE-dCas9-BFP-VP64

Plasmid

#196717

Purpose

CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

Depositor

Bernhard Schmierer

Insert

dCas9-TagBFP-VP64

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mutation

D10A, N863A

Promoter

EF1a

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBS185 CMV-Cre

Plasmid

#11916

Purpose

Mammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoter

Depositor

Brian Sauer

Article

Sauer et al New Biol. 1990 May . 2(5):441-9.

Insert

cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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