163,297 results

1. pK18sB

   Plasmid

   #177838

   Purpose

   Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

   Type

   Empty backbone

   Use

   Bacterial gene replacement

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCEP4-myc-ACE2

   Plasmid

   #141185

   Purpose

   Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPYL-ABI

   Plasmid

   #233650

   Purpose

   Cellular colocalization assay (PYL/ABI) for the CIP OP (opabactin) or the CIP ABA (abscisic acid)

   Depositor

   Richard Wombacher

   Article

   Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

   Insert

   TOMM20-mCherry-PYL-P2A-eGFP-ABI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTriEx-mCherry-LOV2

   Plasmid

   #81041

   Purpose

   Mammalian or insect expression for wt LOV2

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV2
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV, p10

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBBR15.2-2H12.D11opt-scarRef

   Plasmid

   #221150

   Purpose

   cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen2.1
   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   Constitutive and None (same as cdGreen2.1)

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGTMV

   Plasmid

   #118755

   Purpose

   Binary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.

   Depositor

   Jose-Antonio Daros

   Article

   Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

   Insert

   Tobacco mosaic virus
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sfGFP-pBAD

   Plasmid

   #54519

   Purpose

   Localization: Protein Expression Vector , Excitation: 485, Emission: 507

   Depositor

   Michael Davidson, Geoffrey Waldo

   Article

   Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

   Type

   Empty backbone

   Use

   Tags

   6xHis and SfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pHR hEF1A-BFP-P2A-rtTA-WPRE

   Plasmid

   #223288

   Purpose

   A lentiviral vector expressing BFP and the TetON transcription factor rtTA.

   Depositor

   Jared Toettcher

   Article

   Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

   Insert

   BFP-P2A-rtTA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-mtagBFP

   Plasmid

   #89685

   Purpose

   pCAG promoter driving mtagBFP

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   mtagBFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pF CAG luc IRES neo

    Plasmid

    #98294

    Purpose

    For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Inserts

    Firefly luciferase
    IRES
    Neomycin resistance gene (NeoR)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVC_CRISPR_Cat

    Plasmid

    #120368

    Purpose

    Reporter plasmid for full-site selection assay

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    CRISPR_Cat
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFGC5941-PacI-ath-STTM160

    Plasmid

    #84085

    Purpose

    target miRNA160 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM160
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Col18a1_pCSdest

    Plasmid

    #53930

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    Col18a1 (Col18a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670) (AAV9)

    Viral Prep

    #100061-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670) (#100061). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670) plasmid DNA. EFIa-driven, Cre-dependent, C1V1 (t/t) fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    mCherry (Cre-dependent)

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

15. Humagne Set C

    Pooled Library

    #172650

    Purpose

    Set C contains best-ranked guides 1 and 4.

    Depositor

    David Root, John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Expression

    Mammalian

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA-Flag-Tet3

    Plasmid

    #60940

    Purpose

    Expresses Tet3 in mammalian cells

    Depositor

    Yi Zhang

    Article

    Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

    Insert

    Ten-Eleven Translocation 3
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX330-PITCh-H2BC11

    Plasmid

    #207755

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-FLEX-Arch-GFP (AAV1)

    Viral Prep

    #22222-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-FLEX-Arch-GFP (#22222). In addition to the viral particles, you will also receive purified AAV-FLEX-Arch-GFP plasmid DNA. CAG-driven cre-dependent Arch-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Promoter

    CAG

    Tags

    GFP (Cre-dependent)

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pLVX-TetOne-Puro-p21

    Plasmid

    #171122

    Purpose

    Doxycycline inducible expression of p21

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    p21 (CDKN1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Bx

    Plasmid

    #51552

    Purpose

    Expresses Bxb1 integrase in mammalian cells.

    Depositor

    Michele Calos

    Article

    Zhu et al Nucleic Acids Res. 2013 Dec 4.

    Insert

    Bxb1 integrase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge