167,564 results

1. px459V2.0_Conc2

   Plasmid

   #134451

   Purpose

   Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. iRFP670-KASH2

   Plasmid

   #187021

   Purpose

   Inducible disruption of LINC Complex

   Depositor

   Jan Lammerding

   Insert

   KASH2
   

   Tags

   iRFP670

   Expression

   Mammalian

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO-MCS

   Plasmid

   #185594

   Purpose

   Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFH2.32_LV-mCherry-MS2x8-BCS

   Plasmid

   #205539

   Purpose

   Express mCherry-MS2x8 with barcode

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX-RCC1-HA

   Plasmid

   #106938

   Purpose

   Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

   Depositor

   Patrizia Lavia

   Article

   DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

   Insert

   human RCC1-HA (RCC1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1-FH-AGO2-WT

   Plasmid

   #91978

   Purpose

   Expresses FLAG-HA-AGO2 (WT)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. HIP-flash (HOP-flash mutant)

   Plasmid

   #83466

   Purpose

   Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

   Depositor

   Barry Gumbiner

   Article

   Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

   Insert

   Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
   

   Use

   Luciferase

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLY100-RetroEFS-HER2-28BBz-WPRE

   Plasmid

   #192201

   Purpose

   Retro-HER2CAR

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Retro-HER2CAR (ERBB2 Synthetic)
   

   Use

   Retroviral; Mammalian expression

   Mutation

   NA

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-NLS-GFP

   Plasmid

   #104061

   Purpose

   AAV expression of nuclear localized GFP with SV40 NLS

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Has Service

   AAV PHP.eB

   Insert

   GFP
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-BICD2

    Plasmid

    #164626

    Purpose

    Expresses BICD2 in mammalian cells with a GFP tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    BICD2 (BICD2 Human)
    

    Tags

    Cycle 3 GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-M-Flag-BAP1-C91G

    Plasmid

    #125845

    Purpose

    express mutant BAP1-C91G

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    C91G

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.1(+)-NEMOm

    Plasmid

    #189930

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOs
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. B-gTEMP/pcDNA3

    Plasmid

    #188447

    Purpose

    B-gTEMP for mammalian expression

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-CAG-hrGFP

    Plasmid

    #52344

    Purpose

    AAVS1 donor vector for constitutive gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    hrGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. FAS promoter luciferase

    Plasmid

    #8890

    Depositor

    Bruce Spiegelman

    Article

    Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

    Insert

    Fatty Acid Synthetase Promoter (Fasn Rat, unknown - rat or mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-Bxb1-LP-TC

    Plasmid

    #183754

    Purpose

    AAVS1 targeting construct with the Bxb1 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    loxP-eGFP-attP-Bsd-lox257
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-opto-mRipK3

    Plasmid

    #208783

    Purpose

    Inducibly expresses mouse RipK3 fused to light activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    mCherry mRipK3 Cry2 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Cry2 and mCherry

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG mito-RCaMP1h

    Plasmid

    #105013

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV.tetO.Ascl1.U6.shRest1.U6.shRest2

    Plasmid

    #234846

    Purpose

    3rd generation lentiviral vector, expresses Ascl1 under control of TetON promoter and two shREST sequences under control of U6 promoters

    Depositor

    Malin Parmar

    Article

    Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1 Blast NT shRNA

    Plasmid

    #123306

    Purpose

    Expression of a non-targeting shRNA

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    Non-targeting shRNA
    

    Use

    Lentiviral and RNAi

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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