|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37277||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGhader Bashiri
- Backbone size (bp) 4921
Vector typeBacterial Expression ; mycobacteria shuttle vector
- Promoter T7
/ Fusion Proteins
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYUB28b was a gift from Ted Baker (Addgene plasmid # 37277 ; http://n2t.net/addgene:37277 ; RRID:Addgene_37277)
For your References section:Metabolic engineering of cofactor F420 production in Mycobacterium smegmatis. Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN. PLoS One. 2010 Dec 29;5(12):e15803. 10.1371/journal.pone.0015803 PubMed 21209917