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(Plasmid #42191)


Item Catalog # Description Quantity Price (USD)
Plasmid 42191 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Daugelat et al 2003
  • Backbone size (bp) 6900
  • Modifications to backbone
    Vector backbone corresponds to the pSD26 plasmid [Daugelat et al 2003] with two modifications: (i) deleting the six initial codons of acetamidase, (ii) inserting a multiple cloning site (MCS) consisting of restriction endonuclease sites, NcoI, KpnI, BamHI, EcoRI, EcoRV and HindIII. Middle:
  • Vector type
    Bacterial Expression
  • Promoter ColE1
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Unknown

Resource Information

Depositor Comments

Vector constructed by Dr. Arie Geerlof at EMBL-Hamburg (now at Helmholtz Zentrum München)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMyNT was a gift from Annabel Parret & Matthias Wilmanns (Addgene plasmid # 42191 ; ; RRID:Addgene_42191)
  • For your References section:

    The pMy vector series: A versatile cloning platform for the recombinant production of mycobacterial proteins in Mycobacterium smegmatis. Beckham KSH, Staack S, Wilmanns M, Parret AHA. Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962. 10.1002/pro.3962 PubMed 33006405